Rab-GDI 1 antibody
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- Target
- Rab-GDI 1
- Reactivity
- Cow, Hamster, Human, Mouse, Rat
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
- Specificity
- Recognizes Rab-GDI-alpha and -beta.
- Purification
- purified IgG
- Immunogen
- Purified Rab-GDI of bovine brain.
- Clone
- 81-2
- Isotype
- IgG1
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- Application Notes
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WB: 1 : 1000 up to 1 : 10000 (AP staining)
IHC: not tested yet - Comment
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Immunoprecipitates Rab-GDI complexes.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Handling Advice
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
- Storage
- -20 °C
- Storage Comment
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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Isoprenylcysteine carboxylmethyltransferase function is essential for RAB4A-mediated integrin β3 recycling, cell migration and cancer metastasis." in: Oncogene, Vol. 36, Issue 41, pp. 5757-5767, (2017) (PubMed).
: "The GTPase Rab26 links synaptic vesicles to the autophagy pathway." in: eLife, Vol. 4, pp. e05597, (2015) (PubMed).
: "Ear2 deletion causes early memory and learning deficits in APP/PS1 mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 26, pp. 8845-54, (2014) (PubMed).
: "α-Synuclein membrane association is regulated by the Rab3a recycling machinery and presynaptic activity." in: The Journal of biological chemistry, Vol. 288, Issue 11, pp. 7438-49, (2013) (PubMed).
: "Mover is a homomeric phospho-protein present on synaptic vesicles." in: PLoS ONE, Vol. 8, Issue 5, pp. e63474, (2013) (PubMed).
: "RIM proteins tether Ca2+ channels to presynaptic active zones via a direct PDZ-domain interaction." in: Cell, Vol. 144, Issue 2, pp. 282-95, (2011) (PubMed).
: "Alpha-synuclein promotes SNARE-complex assembly in vivo and in vitro." in: Science (New York, N.Y.), Vol. 329, Issue 5999, pp. 1663-7, (2010) (PubMed).
: "Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 40, pp. 13441-53, (2010) (PubMed).
: "Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).
: "CSPα promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity." in: Nature cell biology, Vol. 13, Issue 1, pp. 30-9, (2010) (PubMed).
: "SynCAMs organize synapses through heterophilic adhesion." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 46, pp. 12516-30, (2007) (PubMed).
: "A dual function for Munc-18 in exocytosis of PC12 cells." in: The European journal of neuroscience, Vol. 21, Issue 9, pp. 2419-32, (2005) (PubMed).
: "Rap1-induced p38 mitogen-activated protein kinase activation facilitates AMPA receptor trafficking via the GDI.Rab5 complex. Potential role in (S)-3,5-dihydroxyphenylglycene-induced long term ..." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12286-92, (2004) (PubMed).
: "Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules." in: Molecular and cellular biology, Vol. 22, Issue 18, pp. 6487-97, (2002) (PubMed).
: "Binding of Rab3A to synaptic vesicles." in: The Journal of biological chemistry, Vol. 275, Issue 13, pp. 9433-40, (2000) (PubMed).
: "Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses." in: The European journal of neuroscience, Vol. 11, Issue 4, pp. 1335-48, (1999) (PubMed).
: "Rabphilin knock-out mice reveal that rabphilin is not required for rab3 function in regulating neurotransmitter release." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 14, pp. 5834-46, (1999) (PubMed).
: "
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Isoprenylcysteine carboxylmethyltransferase function is essential for RAB4A-mediated integrin β3 recycling, cell migration and cancer metastasis." in: Oncogene, Vol. 36, Issue 41, pp. 5757-5767, (2017) (PubMed).
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- Target
- Rab-GDI 1
- Background
- Synonyms: GDI 1
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