For immunohistochemistry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Restrictions
For Research Use only
Buffer
PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Jurisicova, Taniuchi, Li, Shang, Antenos, Detmar, Xu, Matikainen, Benito Hernández, Nunez, Casper: "Maternal exposure to polycyclic aromatic hydrocarbons diminishes murine ovarian reserve via induction of Harakiri." in: The Journal of clinical investigation, Vol. 117, Issue 12, pp. 3971-8, (2007) (PubMed).
Maisonnette, Simon, Hennion, Pichon: "Selective immunoclean-up followed by liquid chromatography for the monitoring of a biomarker of exposure to polycyclic aromatic hydrocarbons in urine at the ng l-1 level." in: Journal of chromatography. A, Vol. 1120, Issue 1-2, pp. 185-93, (2006) (PubMed).
Rybicki, Rundle, Savera, Sankey, Tang: "Polycyclic aromatic hydrocarbon-DNA adducts in prostate cancer." in: Cancer research, Vol. 64, Issue 24, pp. 8854-9, (2004) (PubMed).
Zhang, Weksler, Wang, Schwartz, Santella: "Immunohistochemical detection of polycyclic aromatic hydrocarbon-DNA damage in human blood vessels of smokers and non-smokers." in: Atherosclerosis, Vol. 140, Issue 2, pp. 325-31, (1999) (PubMed).
Santella, Lin, Cleveland, Weinstein: "Monoclonal antibodies to DNA modified by a benzo[a]pyrene diol epoxide." in: Carcinogenesis, Vol. 5, Issue 3, pp. 373-7, (1984) (PubMed).
Target
BPDE-DNA
(Benzo[a]pyrenediol Epoxide Modified DNA (BPDE-DNA))
A number of chemicals, including polycyclic aromatic hydrocarbons (PAHs), have been shown to bind to DNA. This DNA damage can occur both early and late in the malignant process, thereby acting as an initiator and assisting in the progression of tumors. PAHs are released into the environment following incomplete combustion of organic materials. The most common sources of PAHs are from smoking and from consuming broiled or grilled foods. Human exposure to PAHs comes from various occupational, environmental, dietary and medicinal sources. Benzo[a]pyrene is a representative PAH. Antibodies to benzo[a]pyrenediol-epoxide modified DNA (BPDE-DNA) can be used to identify polycyclic aromatic hydrocarbon (PAH)-DNA adducts. Exposure to this group of compounds is believed to be carcinogenic. The monoclonal antibody 5D11 recognizes BPDE-I-DNA (PAH-DNA). Immunogen BPDE-I-DNA complexed with methylated BSA