KDM5B antibody (AA 231-319)

Catalog No. ABIN1327126

Product Details anti-KDM5B Antibody

Target: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Binding Specificity: AA 231-319

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KDM5B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Mouse monoclonal antibody raised against a partial recombinant KDM5B.

Sequence: AKRMRAEAMN IKIEPEETTE ARTHNLRRRM GCPTPKCENE KEMKSSIKQE PIERKDYIVE NEKEKPKSRS KKATNAVDLY VCLLCGSGN

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: KDM5B (NP_006609, 231 a.a. ~ 319 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 1G10

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-KDM5B antibody (ABIN1327126)

Mitra, Das, Huynh, Jones: "Jumonji/ARID1 B (JARID1B) protein promotes breast tumor cell cycle progression through epigenetic repression of microRNA let-7e." in: The Journal of biological chemistry, Vol. 286, Issue 47, pp. 40531-5, (2011) (PubMed).

Hayami, Yoshimatsu, Veerakumarasivam, Unoki, Iwai, Tsunoda, Field, Kelly, Neal, Yamaue, Ponder, Nakamura, Hamamoto: "Overexpression of the JmjC histone demethylase KDM5B in human carcinogenesis: involvement in the proliferation of cancer cells through the E2F/RB pathway." in: Molecular cancer, Vol. 9, pp. 59, (2010) (PubMed).

Xiang, Zhu, Han, Ye, Xu, Peng, Ma, Yu, Lin, Chen, Chen: "JARID1B is a histone H3 lysine 4 demethylase up-regulated in prostate cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 49, pp. 19226-31, (2007) (PubMed).

Target Details for KDM5B

Target: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Alternative Name: KDM5B (KDM5B Products)

Background: Full Gene Name: jumonji, AT rich interactive domain 1B
Synonyms: FLJ10538,FLJ12459,FLJ12491,FLJ16281,FLJ23670,KDM5B,PLU-1,PUT1,RBBP2H1A

Gene ID: 10765

NCBI Accession: NM_006618

Pathways: Warburg Effect