FHL1 antibody (Internal Region)

Catalog No. ABIN185289

Product Details anti-FHL1 Antibody

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Binding Specificity: Internal Region

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This FHL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Fhl1/ Slim1 (mouse)

Sequence: CNKRFVFHNE QVY

Specificity: This antibody is expected to recognize Mouse NP_001070830.1 iso 2 , NP_001274729.1 iso 3 and Rat NP_001029098.1 iso 1, NP_001258128.1 iso 3.

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence CNKRFVFHNEQVY, from the internal region of the protein sequence according to NP_001440.1, NP_001153171.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 32 kDa band observed in Mouse and Rat Skeletal Muscle and in Rat Lung lysates (calculated MW of 31.9 kDa according to Mouse NP_001274729.1 and Rat NP_001258128.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at r

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-FHL1 antibody (ABIN185289)

Tang, Lai, Hammond, Roth, Yang, Guo, Gao: "Adenylyl cyclase 6 deletion reduces left ventricular hypertrophy, dilation, dysfunction, and fibrosis in pressure-overloaded female mice." in: Journal of the American College of Cardiology, Vol. 55, Issue 14, pp. 1476-86, (2010) (PubMed).

Gramolini, Kislinger, Alikhani-Koopaei, Fong, Thompson, Isserlin, Sharma, Oudit, Trivieri, Fagan, Kannan, Higgins, Huedig, Hess, Arab, Seidman, Seidman, Frey, Perry, Backx, Liu, MacLennan, Emili: "Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 519-33, (2008) (PubMed).

Target Details for FHL1

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Alternative Name: FHL1 (FHL1 Products)

Background: FHL1, SLIM1, KYO-T, RP11-535K18.1, FHL1A, four and a half LIM domains 1, Four-and-a-half LIM domains 1, skeletal muscle LIM-protein1, FHL1B, KYOT, MGC111107, XMPMA, SLIMMER, LIM protein SLIMMER, four-and-a-half Lin11, Isl-1 and Mec-3 domains 1, skeletal m

Gene ID: 14199, 25177

NCBI Accession: NP_001440, NP_001153171