DARPP32 antibody (pThr34)

Catalog No. ABIN372606

Product Details anti-DARPP32 Antibody

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Binding Specificity: pThr34

Reactivity: Human, Mouse, Rat, Cow, Chicken, Dog, Non-Human Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DARPP32 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Specific for the ~32k DARPP-32 protein phosphorylated at Thr34. Immunolabeling is blocked by Lambda-phosphatase treatment.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse and non-Human Primates.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr34 of rat DARPP-32.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-DARPP32 antibody (ABIN372606)

Marazziti, Di Pietro, Mandillo, Golini, Matteoni, Tocchini-Valentini: "Absence of the GPR37/PAEL receptor impairs striatal Akt and ERK2 phosphorylation, DeltaFosB expression, and conditioned place preference to amphetamine and cocaine." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 6, pp. 2071-81, (2011) (PubMed).

Maiya, Zhou, Norris, Kreek, Strickland: "Tissue plasminogen activator modulates the cellular and behavioral response to cocaine." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 6, pp. 1983-8, (2009) (PubMed).

Niculescu, Perrine, Miller, Ehrlich, Unterwald: "Trk: a neuromodulator of age-specific behavioral and neurochemical responses to cocaine in mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 5, pp. 1198-207, (2008) (PubMed).

Target Details for DARPP32

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Alternative Name: DARPP32 (PPP1R1B Products)

Synonyms: darpp32 antibody, darpp-32 antibody, Darpp-32 antibody, Darpp32 antibody, DARPP-32 antibody, DARPP32 antibody, AU040756 antibody, protein phosphatase 1 regulatory inhibitor subunit 1B antibody, protein phosphatase 1, regulatory (inhibitor) subunit 1B antibody, protein phosphatase 1 regulatory inhibitor subunit 1B S homeolog antibody, ppp1r1b antibody, PPP1R1B antibody, Ppp1r1b antibody, ppp1r1b.S antibody

Background: DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002).Synonyms: DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B

Gene ID: 84152

NCBI Accession: NP_001229393

UniProt: Q9UD71