Alpha, beta Synuclein (AA 2-25), (N-Term) antibody

Catalog No. ABIN1742282

Product Details

Target: Alpha, beta Synuclein

Binding Specificity: AA 2-25, N-Term

Reactivity: Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Recognizes alpha synuclein and beta synuclein.

Purification: antiserum

Immunogen: Synthetic peptide DVFMKGLSKAKEGVVAAAEKTKQG (aa 2-25 in alpha synuclein), coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 500
IHC: 1 : 500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Siebert, Kahle, Kramer, Isik, Schlüter, Schulz-Schaeffer, Brück: "Over-expression of alpha-synuclein in the nervous system enhances axonal degeneration after peripheral nerve lesion in a transgenic mouse strain." in: Journal of neurochemistry, Vol. 114, Issue 4, pp. 1007-18, (2010) (PubMed).

Tong, Wong, Guttman, Ang, Forno, Shimadzu, Rajput, Muenter, Kish, Hornykiewicz, Furukawa: "Brain alpha-synuclein accumulation in multiple system atrophy, Parkinson's disease and progressive supranuclear palsy: a comparative investigation." in: Brain : a journal of neurology, Vol. 133, Issue Pt 1, pp. 172-88, (2010) (PubMed).

Cole, Dieuliis, Leo, Mitchell, Nussbaum: "Mitochondrial translocation of alpha-synuclein is promoted by intracellular acidification." in: Experimental cell research, Vol. 314, Issue 10, pp. 2076-89, (2008) (PubMed).

Colapinto, Mila, Giraudo, Stefanazzi, Molteni, Rossetti, Bergamasco, Lopiano, Fasano: "alpha-Synuclein protects SH-SY5Y cells from dopamine toxicity." in: Biochemical and biophysical research communications, Vol. 349, Issue 4, pp. 1294-300, (2006) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Steinacker, Schwarz, Reim, Brechlin, Jahn, Kratzin, Aitken, Wiltfang, Aguzzi, Bahn, Baxter, Brose, Otto: "Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein." in: Molecular and cellular biology, Vol. 25, Issue 4, pp. 1339-46, (2005) (PubMed).

Target Details

Target: Alpha, beta Synuclein

Alternative Name: alpha/beta Synuclein

Background: Synonyms: alpha-Synuclein, beta-Synuclein