IL-33 Protein (AA 112-270) (His tag)

Catalog No. ABIN1344110

Product Details

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 112-270

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This IL-33 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Specificity: Binds to human ST2.

Cross-Reactivity: Human

Characteristics: Human IL-33 (aa 112-270) is fused at the C-terminus to a His-tag.

Purity: >90 % (SDS-PAGE)

Sterility: 0.2 μm filtered

Endotoxin Level: <1EU/μg purified protein (LAL test, Lonza).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Activates human ST2-dependent NF-kappaB pathway.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.2μm-filtered solution in 55 mM TRIS-Cl, pH 8.2, containing 150 mM NaCl.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 3 months

References

Suzukawa, Koketsu, Iikura, Nakae, Matsumoto, Nagase, Saito, Matsushima, Ohta, Yamamoto, Yamaguchi: "Interleukin-33 enhances adhesion, CD11b expression and survival in human eosinophils." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 88, Issue 11, pp. 1245-53, (2008) (PubMed).

Suzukawa, Iikura, Koketsu, Nagase, Tamura, Komiya, Nakae, Matsushima, Ohta, Yamamoto, Yamaguchi: "An IL-1 cytokine member, IL-33, induces human basophil activation via its ST2 receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 9, pp. 5981-9, (2008) (PubMed).

Iikura, Suto, Kajiwara, Oboki, Ohno, Okayama, Saito, Galli, Nakae: "IL-33 can promote survival, adhesion and cytokine production in human mast cells." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 87, Issue 10, pp. 971-8, (2007) (PubMed).

Target Details

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: IL-33 (IL33 Products)

Synonyms: IL33 Protein, C9orf26 Protein, DVS27 Protein, IL1F11 Protein, NF-HEV Protein, NFEHEV Protein, RP11-575C20.2 Protein, 9230117N10Rik Protein, Il-33 Protein, Il1f11 Protein, RGD1311155 Protein, interleukin 33 Protein, IL33 Protein, Il33 Protein

Background: Interleukin-33 (IL-33, HF-NEV, IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30 kDa human IL33 is converted by CASP1 to a 18 kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38 (MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.

Molecular Weight: ~18kDa (SDS-PAGE)

UniProt: Q2YEJ5

Pathways: Production of Molecular Mediator of Immune Response