NEFL antibody

Catalog No. ABIN969548

Product Details anti-NEFL Antibody

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purification: purified

Immunogen: Purified recombinant fragment of human NEFL expressed in E. coli.

Clone: 1H3

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: 4°C, -20°C for long term storage

References for anti-NEFL antibody (ABIN969548)

Lee, Kim, Chung, Kim, Kwon, Min, Chang: "The effect of rod domain A148V mutation of neurofilament light chain on filament formation." in: BMB reports, Vol. 41, Issue 12, pp. 868-74, (2009) (PubMed).

Abe, Numakura, Saito, Koide, Oka, Honma, Kishikawa, Hayasaka: "Neurofilament light chain polypeptide gene mutations in Charcot-Marie-Tooth disease: nonsense mutation probably causes a recessive phenotype." in: Journal of human genetics, Vol. 54, Issue 2, pp. 94-7, (2009) (PubMed).

Target Details for NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: NEFL (NEFL Products)

Background:

Description: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.

Aliases: NFL, NF-L, NF68, CMT1F, CMT2E

Molecular Weight: 62 kDa

Gene ID: 4747

HGNC: 4747