EEA1 antibody (AA 2-13)

Catalog No. ABIN1742484

Product Details anti-EEA1 Antibody

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Binding Specificity: AA 2-13

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Specific for EEA 1.

Purification: antiserum

Immunogen: Synthetic peptide (aa 2-13 in rodent EEA 1) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: 1 : 1000 up to 1 : 2000
IHC-P: 1 : 200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-EEA1 antibody (ABIN1742484)

Pavlos, Grønborg, Riedel, Chua, Boyken, Kloepper, Urlaub, Rizzoli, Jahn: "Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 40, pp. 13441-53, (2010) (PubMed).

Barysch, Aggarwal, Jahn, Rizzoli: "Sorting in early endosomes reveals connections to docking- and fusion-associated factors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 24, pp. 9697-702, (2009) (PubMed).

Geumann, Barysch, Hoopmann, Jahn, Rizzoli: "SNARE function is not involved in early endosome docking." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5327-37, (2008) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Target Details for EEA1

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Alternative Name: EEA 1 (EEA1 Products)

Synonyms: MST105 antibody, MSTP105 antibody, ZFYVE2 antibody, im:7151048 antibody, si:ch211-276p10.2 antibody, A430109M19Rik antibody, B230358H09Rik antibody, early endosome antigen 1 antibody, early endosome antigen 1 S homeolog antibody, EEA1 antibody, eea1 antibody, Eea1 antibody, eea1.S antibody

Pathways: Toll-Like Receptors Cascades