Adenosine A2a Receptor antibody (C-Term)

Catalog No. ABIN185549

Product Details anti-Adenosine A2a Receptor Antibody

Target: Adenosine A2a Receptor (ADORA2A)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Macaque

Host: Goat

Clonality: Polyclonal

Conjugate: This Adenosine A2a Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: Adenosine A2a Receptor

Sequence: HELKGVCPEP PGLDD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence HELKGVCPEPPGLDD, from the C Terminus of the protein sequence according to NP_000666.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: This antibody has been successfully used in Western blot on Macaque: van der Putten C et al. (2009) PMID: 19494284. This antibody has been successfully used in Western blot on Mouse: He X et al. (2013) PMID: 23712033, and Zhao L et al. (201

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Adenosine A2a Receptor antibody (ABIN185549)

van der Putten, Zuiderwijk-Sick, van Straalen, de Geus, Boven, Kondova, IJzerman, Bajramovic: "Differential expression of adenosine A3 receptors controls adenosine A2A receptor-mediated inhibition of TLR responses in microglia." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 12, pp. 7603-12, (2009) (PubMed).

Target Details for Adenosine A2a Receptor

Target: Adenosine A2a Receptor (ADORA2A)

Alternative Name: ADORA2A (ADORA2A Products)

Synonyms: ADORA2A antibody, adora2a.1 antibody, zgc:194773 antibody, A2aR antibody, ADORA2 antibody, RDC8 antibody, A2AAR antibody, AA2AR antibody, A2ar antibody, ADENO antibody, Adora2l1 antibody, adora2a.2 antibody, zgc:193802 antibody, zgc:193822 antibody, adenosine A2a receptor antibody, adenosine A2a receptor a antibody, adenosine A2a receptor L homeolog antibody, adenosine A2a receptor b antibody, ADORA2A antibody, Adora2a antibody, adora2aa antibody, adora2a.L antibody, adora2a antibody, adora2ab antibody

Background: ADORA2A, adenosine A2a receptor , HGNC:263, ADORA2, RDC8, hA2aR , adenosine A2 receptor, adenosine receptor subtype A2a

Gene ID: 135

NCBI Accession: NP_000666

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints