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HLA-DR antibody

HLA-DR Reactivity: Human, Dog, Non-Human Primate FACS, WB, IP, IHC (p), IHC (fro), ICC Host: Mouse Monoclonal L243 unconjugated
Catalog No. ABIN1027699
  • Target See all HLA-DR products
    HLA-DR
    Reactivity
    • 124
    • 20
    • 18
    • 17
    • 17
    • 14
    • 11
    • 8
    • 5
    • 4
    • 3
    • 3
    • 2
    Human, Dog, Non-Human Primate
    Host
    • 105
    • 12
    • 10
    Mouse
    Clonality
    • 121
    • 5
    Monoclonal
    Conjugate
    • 40
    • 16
    • 16
    • 10
    • 8
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HLA-DR antibody is un-conjugated
    Application
    • 110
    • 35
    • 28
    • 18
    • 17
    • 11
    • 9
    • 8
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
    Specificity
    The mouse monoclonal antibody L243 recognizes specifically an extracellular epitope on HLA-DR molecules, both peptide-loaded and empty.
    Cross-Reactivity (Details)
    Human, Non-Human Primates, Canine (Dog)
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Human B lymphocytes
    Clone
    L243
    Isotype
    IgG2a kappa
  • Application Notes
    Flow cytometry: Recommended dilution: 1-4 μg/mL.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Ivanov, Beers, Walshe, Honeychurch, Alduaij, Cox, Potter, Murray, Chan, Klymenko, Erenpreisa, Glennie, Illidge, Cragg: "Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells." in: The Journal of clinical investigation, Vol. 119, Issue 8, pp. 2143-59, (2009) (PubMed).

    De Gassart, Camosseto, Thibodeau, Ceppi, Catalan, Pierre, Gatti: "MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 9, pp. 3491-6, (2008) (PubMed).

    Bouillon, El Fakhry, Girouard, Khalil, Thibodeau, Mourad: "Lipid raft-dependent and -independent signaling through HLA-DR molecules." in: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 7099-107, (2003) (PubMed).

    Kalka-Moll, Tzianabos, Bryant, Niemeyer, Ploegh, Kasper: "Zwitterionic polysaccharides stimulate T cells by MHC class II-dependent interactions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 11, pp. 6149-53, (2002) (PubMed).

  • Target
    HLA-DR
    Alternative Name
    HLA-DR (HLA-DR Products)
    Background
    HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.
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