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GAD65 Protein (AA 1-585) (GST tag)

GAD2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Catalog No. ABIN1354671
  • Target See all GAD65 (GAD2) Proteins
    GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-585
    Origin
    • 7
    • 5
    • 3
    • 1
    Human
    Source
    • 6
    • 3
    • 2
    • 2
    • 1
    Wheat germ
    Purification tag / Conjugate
    This GAD65 protein is labelled with GST tag.
    Application
    Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
    Purpose
    GAD2 (Human) Recombinant Protein (P02)
    Sequence
    MASPGSGFWS FGSEDGSGDS ENPGTARAWC QVAQKFTGGI GNKLCALLYG DAEKPAESGG SQPPRAAARK AACACDQKPC SCSKVDVNYA FLHATDLLPA CDGERPTLAF LQDVMNILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA DQPQNLEEIL MHCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA MMIARFKMFP EVKEKGMAAL PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI LIKCDERGKM IPSDLERRIL EAKQKGFVPF LVSATAGTTV YGAFDPLLAV ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV PLQCSALLVR EEGLMQNCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV DVFKLWLMWR AKGTTGFEAH VDKCLELAEY LYNIIKNREG YEMVFDGKPQ HTNVCFWYIP PSLRTLEDNE ERMSRLSKVA PVIKARMMEY GTTMVSYQPL GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL
    Characteristics
    Human GAD2 full-length ORF ( NP_000809.1, 1 a.a. - 585 a.a.) recombinant protein with GST-tag at N-terminal.
    Purification
    in vitro wheat germ expression system
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Preparation method: in vitro, wheat germ expression system
    Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

    Restrictions
    For Research Use only
  • Buffer
    50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
    Handling Advice
    Aliquot to avoid repeated freezing and thawing.
    Storage
    -80 °C
    Storage Comment
    Best use within three months from the date of receipt of this protein.
  • Grupillo, Gualtierotti, He, Sisino, Bottino, Rudert, Trucco, Fan: "Essential roles of insulin expression in Aire+ tolerogenic dendritic cells in maintaining peripheral self-tolerance of islet ?-cells." in: Cellular immunology, Vol. 273, Issue 2, pp. 115-23, (2012) (PubMed).

    Fan, Rudert, Grupillo, He, Sisino, Trucco: "Thymus-specific deletion of insulin induces autoimmune diabetes." in: The EMBO journal, Vol. 28, Issue 18, pp. 2812-24, (2009) (PubMed).

  • Target
    GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
    Alternative Name
    GAD2 (GAD2 Products)
    Synonyms
    GAD65 Protein, gad65 Protein, F12P19.12 Protein, F12P19_12 Protein, GLUTAMATE DECARBOXYLASE 2 Protein, glutamate decarboxylase 2 Protein, zgc:112198 Protein, 6330404F12Rik Protein, GAD(65) Protein, Gad-2 Protein, glutamate decarboxylase 2 Protein, glutamate decarboxylase 1 Protein, glutamic acid decarboxylase 2 Protein, GAD2 Protein, PTRG_06148 Protein, LOC9324534 Protein, Gad2 Protein, GAD1 Protein, gad2 Protein
    Background
    Full Gene Name: glutamate decarboxylase 2 (pancreatic islets and brain, 65 kDa)
    Synonyms: GAD65,MGC161605,MGC161607
    Gene ID
    2572
    NCBI Accession
    NM_000818
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