USP2 antibody (C-Term)

Catalog No. ABIN388879

Product Details anti-USP2 Antibody

Target: USP2 (Ubiquitin Specific Peptidase 2 (USP2))

Binding Specificity: AA 508-538, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: B, M, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This USP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 508-538 amino acids from the C-terminal region of human USP2.

Clone: RB4417

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. IHC-P: 1:50~100. IHC-P: 1:50~100. IHC-P: 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-USP2 antibody (ABIN388879)

Kitamura, Ishino, Shimamoto, Okabe, Miyamoto, Takahashi, Miyoshi: "Ubiquitin-Specific Protease 2 Modulates the Lipopolysaccharide-Elicited Expression of Proinflammatory Cytokines in Macrophage-like HL-60 Cells." in: Mediators of inflammation, Vol. 2017, pp. 6909415, (2018) (PubMed).

Ren, Zhao, Liu, Yuan, Cheng, Zuo, Qian, Liu, Guo, Zhang, Wang, Qian, Li, Ge, Dai, Xiong, Zheng: "Deubiquitinase USP2a Sustains Interferons Antiviral Activity by Restricting Ubiquitination of Activated STAT1 in the Nucleus." in: PLoS pathogens, Vol. 12, Issue 7, pp. e1005764, (2017) (PubMed).

Davis, Pragani, Fox, Shen, Parmar, Gaudiano, Liu, Tanega, McGee, Hall, McKnight, Shinn, Nelson, Chattopadhyay, DAndrea, Auld, DeLucas, Li, Boxer, Simeonov: "Small Molecule Inhibition of the Ubiquitin-specific Protease USP2 Accelerates cyclin D1 Degradation and Leads to Cell Cycle Arrest in Colorectal Cancer and Mantle Cell Lymphoma Models." in: The Journal of biological chemistry, Vol. 291, Issue 47, pp. 24628-24640, (2017) (PubMed).

Boustani, Khoshnood, Nikpasand, Taleshi, Ahmadi, Yahaghi, Goudarzi: "Overexpression of ubiquitin-specific protease 2a (USP2a) and nuclear factor erythroid 2-related factor 2 (Nrf2) in human gliomas." in: Journal of the neurological sciences, Vol. 363, pp. 249-52, (2016) (PubMed).

Zhang, Zhao, Zhang, Zhao, Gao: "Ubiquitin-specific protease 2b negatively regulates IFN-? production and antiviral activity by targeting TANK-binding kinase 1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 193, Issue 5, pp. 2230-7, (2014) (PubMed).

Tong, Buelow, Guha, Rausch, Yin: "USP2a protein deubiquitinates and stabilizes the circadian protein CRY1 in response to inflammatory signals." in: The Journal of biological chemistry, Vol. 287, Issue 30, pp. 25280-91, (2012) (PubMed).

Bedard, Yang, Gregory, Cyr, Suzuki, Yu, Chian, Hermo, OFlaherty, Smith, Clarke, Wing: "Mice lacking the USP2 deubiquitinating enzyme have severe male subfertility associated with defects in fertilization and sperm motility." in: Biology of reproduction, Vol. 85, Issue 3, pp. 594-604, (2011) (PubMed).

Oberfeld, Ruffieux-Daidié, Vitagliano, Pos, Verrey, Staub: "Ubiquitin-specific protease 2-45 (Usp2-45) binds to epithelial Na+ channel (ENaC)-ubiquitylating enzyme Nedd4-2." in: American journal of physiology. Renal physiology, Vol. 301, Issue 1, pp. F189-96, (2011) (PubMed).

Allende-Vega, Sparks, Lane, Saville: "MdmX is a substrate for the deubiquitinating enzyme USP2a." in: Oncogene, Vol. 29, Issue 3, pp. 432-41, (2010) (PubMed).

Shan, Zhao, Gu: "Suppression of cancer cell growth by promoting cyclin D1 degradation." in: Molecular cell, Vol. 36, Issue 3, pp. 469-76, (2009) (PubMed).

Zweitzig, Shcherbik, Haines: "Retraction for D. R. Zweitzig, N. Shcherbik, and D. S. Haines: AAA ATPase P97 and adaptor UBXD1 suppress MDM2 ubiquitination and degradation and promote constitutive P53 turnover." in: Molecular biology of the cell, Vol. 19, Issue 11, pp. 5029, (2008) (PubMed).

Stevenson, Sparks, Allende-Vega, Xirodimas, Lane, Saville: "The deubiquitinating enzyme USP2a regulates the p53 pathway by targeting Mdm2." in: The EMBO journal, Vol. 26, Issue 4, pp. 976-86, (2007) (PubMed).

Target Details for USP2

Target: USP2 (Ubiquitin Specific Peptidase 2 (USP2))

Alternative Name: USP2 (USP2 Products)

Synonyms: UBP41 antibody, USP9 antibody, B930035K21Rik antibody, Ubp41 antibody, UBP-t antibody, UBP46 antibody, ubp52 antibody, ubp66 antibody, zgc:92134 antibody, ubiquitin specific peptidase 2 antibody, ubiquitin specific peptidase 2a antibody, USP2 antibody, Usp2 antibody, usp2a antibody

Background: USP2, an ubiquitin-specific protease, is selectively up regulated in bone by the osteotropic agents PTH, PTHrP and PGE2 and possibly via the PKA/cAMP pathway. It is also thought to play a role in the recycling of ubiquitin by hydrolysis of branched poly-ubiquitin from linear poly-ubiquitin chains, production of free ubiquitin from linear poly-ubiquitin chains and from certain ribosomal ubiquitin fusion proteins.

Molecular Weight: 68072

Gene ID: 9099

NCBI Accession: NP_001230688, NP_004196, NP_741994

UniProt: O75604