TAP Tag antibody

Catalog No. ABIN1842480

Product Details

Target: TAP Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Sequence: CSSGALDYDI PTTASENLYF Q

Specificity: Rabbit Anti-TAP-tag Polyclonal Antibody specifically reacts with fusion proteins containing TAP epitope tag.

Characteristics: Rabbit Anti-TAP-tag Polyclonal Antibody is generated against the common tag, which is composed of a 21-residue peptide, CSSGALDYDIPTTASENLYFQ, derived from the C-terminus of the TAP-tag construct after TEV cleavage.

Purification: Immunoaffinity chromatography

Immunogen: Peptide CSSGALDYDIPTTASENLYFQ conjugated to KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0. 05-0. 2 µg/mL
Western blot: 0. 1-1. 0 µg/mL
Western blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit L00204, C is recommended. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.
Immunoprecipitation (IP):2-10 µg/mg of lysate
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.

Concentration: 0.5 mg/mL

Buffer: lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.

References

Chymkowitch, Eldholm, Lorenz, Zimmermann, Lindvall, Bjørås, Meza-Zepeda, Enserink: "Cdc28 kinase activity regulates the basal transcription machinery at a subset of genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 26, pp. 10450-5, (2012) (PubMed).

Rojas, Moreira-Ramos, Zock-Emmenthal, Urbina, Contreras-Levicoy, Käufer, Maldonado: "Rrn7 protein, an RNA polymerase I transcription factor, is required for RNA polymerase II-dependent transcription directed by core promoters with a HomolD box sequence." in: The Journal of biological chemistry, Vol. 286, Issue 30, pp. 26480-6, (2011) (PubMed).

Shoemaker, Green: "Kinetic analysis reveals the ordered coupling of translation termination and ribosome recycling in yeast." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 51, pp. E1392-8, (2011) (PubMed).

Turner, Malo, Pisclevich, Dash, Davies, Arnason, Harkness: "The Saccharomyces cerevisiae anaphase-promoting complex interacts with multiple histone-modifying enzymes to regulate cell cycle progression." in: Eukaryotic cell, Vol. 9, Issue 10, pp. 1418-31, (2010) (PubMed).

Singla, Hunkapiller, Santos, Seol, Norman, Wakenight, Skarnes, Reiter: "Floxin, a resource for genetically engineering mouse ESCs." in: Nature methods, Vol. 7, Issue 1, pp. 50-2, (2009) (PubMed).

Target Details

Target: TAP Tag

Target Type: Tag

Background: Well-characterized antibodies against short-sequence epitope tags are common in the study of protein expression in several different expression systems. The TAP (tandem affinity purification)-tag allows rapid purification of complexes from a relatively small number of cells even if the researcher does not have prior knowledge of the complexs composition, activity, or function. Combined with mass spectrometry, the TAP-tag strategy enables the identification of proteins interacting with a given target protein1