Magnesium Assay Kit

Catalog No. ABIN1000267

Product Details

Target: Magnesium

Application: Biochemical Assay (BCA)

Sample Type: Serum, Urine, Milk, Food, Beverages

Specificity: 0.1 mg/dL (41 μM)

Characteristics: Sensitive and accurate. Use as little as 5 µL sample. Linear detection range 0.1 mg/dL (41µM) to 3 mg/dL (1.2 mM) Mg 2+ in 96-well plate assay.
Simple and high-throughput. The procedure involves addition of two reagents and measuring OD500nm. Can be readily automated as a high- throughput assay for thousands of samples per day.
Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.
Low interference in biological samples. Assays can be directly performed in serum and urine samples.

Components: Reagent A: 25 mL. Reagent B: 25 mL. EDTA Solution: 2 x 1.5 mL. Standard: 1 mL 10 mg/dL Mg 2+.

Material not included: Pipeting devices and accessories (e.g. 5 µL). Clear bottom 96-well plates (e.g. Corning Costar) and plate reader. Cuvets and spectrophotometer for measuring OD500nm.

Application Details

Application Notes: Direct Assays: Mg 2+ in serum, urine and deproteinated samples (e.g. milk) etc.
Drug Discovery/Pharmacology: effects of drugs on Mg 2+ metabolism.
Food and Beverages: Mg 2+ determination.
Environment: Mg 2+ determination in water and soil.

Comment:

1. EDTA and other Mg 2+ chelators interfere with this assay. This assay can not be applied to plasma samples obtained with EDTA.
2. Sample pretreatment: for milk and other lipid/protein-rich samples, mix equal volumes of sample and 10% trichloroacetic acid. Incubate 5 min at room temperature and pellet precipitates for 2 min at 14,000 rpm in a table centrifuge. Assay the supernatant (dilution factor = 2) using the above procedure.

Protocol: Procedure using 96-well plate:
1. Dilute Standard to 2 mg/dL by mixing 40 µL 10 mg/dL Standard with 160 µL distilled water. Transfer 5 µL diluted standard and samples in duplicate to wells of a clear bottom plate. Diluted standard can be stored at 4°C for future use.
2. Add 200 µL working reagent and tap plate to mix thoroughly.
3. Incubate 2 min at room temperature and read optical density at 500 nm (OD for sample and standard).
4. Add 10 µL EDTA Solution to all sample wells and tap plate to mix thoroughly. Incubate 2 min and read OD at 500nm (OD for blanks).

Procedure using cuvette:
1. Set up test tubes and transfer 25 µL diluted Standard and samples to appropriately labeled tubes.
2. Add 1000 µL working reagent and vortex to mix. Incubate 2 min. Transfer to cuvet and read OD500nm. Add 50 µL EDTA solution, mix well, incubate 2 min and read OD500 nm.

Reagent Preparation:

Prepare enough working reagent by combining equal volumes of Reagent A and Reagent B. Equilibrate to room temperature before use.

Calculation of Results:

Conversions: 1 mg/dL Mg 2+ equals 411 µM, 0.001% or 10 ppm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References

Ullah, Khan, Asif, Khan, Ahmad: "Citrullus colocynthis failed to combat against renal derangements, in spite of its strong antioxidant properties." in: Acta poloniae pharmaceutica, Vol. 70, Issue 3, pp. 533-8, (2013) (PubMed).

Breiderhoff, Himmerkus, Stuiver, Mutig, Will, Meij, Bachmann, Bleich, Willnow, Müller: "Deletion of claudin-10 (Cldn10) in the thick ascending limb impairs paracellular sodium permeability and leads to hypermagnesemia and nephrocalcinosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 35, pp. 14241-6, (2012) (PubMed).

Pachikian, Neyrinck, Deldicque, De Backer, Catry, Dewulf, Sohet, Bindels, Everard, Francaux, Guiot, Cani, Delzenne: "Changes in intestinal bifidobacteria levels are associated with the inflammatory response in magnesium-deficient mice." in: The Journal of nutrition, Vol. 140, Issue 3, pp. 509-14, (2010) (PubMed).

Elizondo, Budi, Parichy: "trpm7 regulation of in vivo cation homeostasis and kidney function involves stanniocalcin 1 and fgf23." in: Endocrinology, Vol. 151, Issue 12, pp. 5700-9, (2010) (PubMed).

LaRusso, Li, Jiang, Uitto: "Elevated dietary magnesium prevents connective tissue mineralization in a mouse model of pseudoxanthoma elasticum (Abcc6(-/-))." in: The Journal of investigative dermatology, Vol. 129, Issue 6, pp. 1388-94, (2009) (PubMed).

Stippler, Fischer, Puccio, Wisniewski, Carson-Walter, Dixon, Walter: "Serum and cerebrospinal fluid magnesium in severe traumatic brain injury outcome." in: Journal of neurotrauma, Vol. 24, Issue 8, pp. 1347-54, (2007) (PubMed).

Kim, Kim, Choi: "Rapid production of milligram quantities of proteins in a batch cell-free protein synthesis system." in: Journal of biotechnology, Vol. 124, Issue 2, pp. 373-80, (2006) (PubMed).

Target Details

Target: Magnesium

Target Type: Element

Background: Quantitative determination of magnesium ion Mg2+ by colorimetric (500nm) method.
Procedure: 10 min.

Magnesium (Mg) is one of the most abundant and essential minerals in mammals. Magnesium is involved in more than 300 biochemical reactions in the body and plays important roles in muscle and nerve functions, heart rhythm, immune system and bone formation. Magnesium deficiency may lead to nausea, fatigue, muscle contractions, hypocalcemia and hypokalemia. Simple, direct and automation-ready procedures for measuring magnesium concentration in biological samples are becoming popular in Research and Drug Discovery. This magnesium assay kit is designed to measure magnesium directly in biological samples without any pretreatment. A calmagite dye in the kit forms a colored complex specifically with magnesium. The intensity of the color, measured at 500 nm, is directly proportional to the magnesium concentration in the sample. The optimized formulation minimizes interference by potential substances.