BMP7 antibody (N-Term)

Catalog No. ABIN2779569

Product Details anti-BMP7 Antibody

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BMP7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: QGKHNSAPMF MLDLYNAMAV EEGGGPGGQG FSYPYKAVFS TQGPPLASLQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 91%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 82%

Characteristics: This is a rabbit polyclonal antibody against BMP7. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human BMP7

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 431 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-BMP7 antibody (ABIN2779569)

Whiteland, Spencer-Harty, Thomas, Davies, Morgan, Kynaston, Bose, Fenn, Lewis, Bodger, Jenkins, Doak: "Putative prognostic epithelial-to-mesenchymal transition biomarkers for aggressive prostate cancer." in: Experimental and molecular pathology, Vol. 95, Issue 2, pp. 220-6, (2013) (PubMed).

Lovric, Chen, Yu, Oliver, Genin, Walsh: "Effects of demineralized bone matrix on tendon-bone healing in an intra-articular rodent model." in: The American journal of sports medicine, Vol. 40, Issue 10, pp. 2365-74, (2012) (PubMed).

Rui, Lui, Rolf, Wong, Lee, Chan: "Expression of chondro-osteogenic BMPs in clinical samples of patellar tendinopathy." in: Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA, Vol. 20, Issue 7, pp. 1409-17, (2012) (PubMed).

Fiaschetti, Castelletti, Zoller, Schramm, Schroeder, Nagaishi, Stearns, Mittelbronn, Eggert, Westermann, Ohgaki, Shalaby, Pruschy, Arcaro, Grotzer: "Bone morphogenetic protein-7 is a MYC target with prosurvival functions in childhood medulloblastoma." in: Oncogene, Vol. 30, Issue 25, pp. 2823-35, (2011) (PubMed).

Filion, Li, Mason-Savas, Kreider, Goldstein, Ayers, Song: "Elastomeric osteoconductive synthetic scaffolds with acquired osteoinductivity expedite the repair of critical femoral defects in rats." in: Tissue engineering. Part A, Vol. 17, Issue 3-4, pp. 503-11, (2011) (PubMed).

Mae, Shirasawa, Yoshie, Sato, Kanoh, Ichikawa, Yokoyama, Yue, Tomotsune, Sasaki: "Combination of small molecules enhances differentiation of mouse embryonic stem cells into intermediate mesoderm through BMP7-positive cells." in: Biochemical and biophysical research communications, Vol. 393, Issue 4, pp. 877-82, (2010) (PubMed).

Yi, Tan, Cho, Wang, Luo, Zhang, Li, Liu: "Regulation of embryonic kidney branching morphogenesis and glomerular development by KISS1 receptor (Gpr54) through NFAT2- and Sp1-mediated Bmp7 expression." in: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17811-20, (2010) (PubMed).

Morrissey, Brown, Pitts, Vessella, Corey: "Bone morphogenetic protein 7 is expressed in prostate cancer metastases and its effects on prostate tumor cells depend on cell phenotype and the tumor microenvironment." in: Neoplasia (New York, N.Y.), Vol. 12, Issue 2, pp. 192-205, (2010) (PubMed).

Target Details for BMP7

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Alternative Name: BMP7 (BMP7 Products)

Background: The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity.The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity.
Alias Symbols: OP-1
Protein Interaction Partner: NOTCH2NL, KRTAP10-3, KRTAP10-8, KRTAP10-5, KRTAP10-7, MTUS2, TRIM27, KRTAP5-9, ACTN4, BMPR2, UBC, SMAD3, SOSTDC1, CHRDL2, GDF7, BMPR1B, NOG, ACVR2B, NCOA3, BMPR1A, ENG, ACVR2A, ACVR1, SMAD1, BMP7,
Protein Size: 431

Molecular Weight: 49 kDa

Gene ID: 655

NCBI Accession: NM_001719, NP_001710

UniProt: P18075

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance