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MAP1LC3A antibody (N-Term)

MAP1LC3A Reactivity: Human, Mouse, Rat WB, IF, IHC (p) Host: Rabbit Polyclonal RB7609 unconjugated
Catalog No. ABIN388464
  • Target See all MAP1LC3A Antibodies
    MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))
    Binding Specificity
    • 22
    • 21
    • 20
    • 15
    • 12
    • 11
    • 11
    • 10
    • 10
    • 8
    • 8
    • 7
    • 7
    • 7
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-30, N-Term
    Reactivity
    • 212
    • 105
    • 86
    • 12
    • 9
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 174
    • 44
    • 2
    Rabbit
    Clonality
    • 165
    • 55
    Polyclonal
    Conjugate
    • 109
    • 18
    • 15
    • 14
    • 14
    • 13
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This MAP1LC3A antibody is un-conjugated
    Application
    • 198
    • 95
    • 74
    • 46
    • 33
    • 31
    • 29
    • 14
    • 14
    • 14
    • 11
    • 10
    • 9
    • 6
    • 5
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
    Immunogen
    This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of human LC3 (APG8a).
    Clone
    RB7609
    Isotype
    Ig Fraction
    Top Product
    Discover our top product MAP1LC3A Primary Antibody
  • Application Notes
    IF: 1:200. WB: 1000. IHC-P: 1:50~100
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
    Expiry Date
    6 months
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    Zhu, Tong, Teh, Wang: "Forkhead box protein O3 transcription factor negatively regulates autophagy in human cancer cells by inhibiting forkhead box protein O1 expression and cytosolic accumulation." in: PLoS ONE, Vol. 9, Issue 12, pp. e115087, (2014) (PubMed).

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  • Target
    MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))
    Alternative Name
    LC3 (MAP1LC3A Products)
    Synonyms
    ATG8E antibody, LC3 antibody, LC3A antibody, MAP1ALC3 antibody, MAP1BLC3 antibody, 1010001H21Rik antibody, 4922501H04Rik antibody, LC3a antibody, MGC69006 antibody, zgc:77094 antibody, MGC89867 antibody, MAP1LC3A antibody, map1lc3a antibody, microtubule associated protein 1 light chain 3 alpha antibody, microtubule-associated protein 1 light chain 3 alpha antibody, microtubule associated protein 1 light chain 3 alpha S homeolog antibody, MAP1LC3A antibody, Map1lc3a antibody, map1lc3a.S antibody, map1lc3a antibody
    Background
    Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
    Molecular Weight
    14272
    Gene ID
    84557
    NCBI Accession
    NP_115903, NP_852610
    UniProt
    Q9H492
    Pathways
    Autophagy
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