Fibroblasts (Reticular) antibody

Catalog No. ABIN2191835

Product Details

Target: Fibroblasts (Reticular)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity (Details): Cross reactivity: Human fibroblasts : Yes

Sterility: 0.2 μm filtered

Clone: ER-TR7

Isotype: IgG2a

Application Details

Application Notes: For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive Spleen control Negative Lymphoid cells control

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References

Alba, Bradshaw, Coughlan, Denby, McDonald, Waddington, Buckley, Greig, Parker, Miller, Wang, Lieber, van Rooijen, McVey, Nicklin, Baker: "Biodistribution and retargeting of FX-binding ablated adenovirus serotype 5 vectors." in: Blood, Vol. 116, Issue 15, pp. 2656-64, (2010) (PubMed).

Svensson, Maroof, Ato, Kaye: "Stromal cells direct local differentiation of regulatory dendritic cells." in: Immunity, Vol. 21, Issue 6, pp. 805-16, (2004) (PubMed).

Nolte, Beliën, Schadee-Eestermans, Jansen, Unger, van Rooijen, Kraal, Mebius: "A conduit system distributes chemokines and small blood-borne molecules through the splenic white pulp." in: The Journal of experimental medicine, Vol. 198, Issue 3, pp. 505-12, (2003) (PubMed).

Kalled, Cutler, Datta, Thomas: "Anti-CD40 ligand antibody treatment of SNF1 mice with established nephritis: preservation of kidney function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 5, pp. 2158-65, (1998) (PubMed).

Van Vliet, Melis, Foidart, Van Ewijk: "Reticular fibroblasts in peripheral lymphoid organs identified by a monoclonal antibody." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 34, Issue 7, pp. 883-90, (1986) (PubMed).

Target Details

Target: Fibroblasts (Reticular)

Background: Monoclonal antibody ER-TR7 recognizes with an intracellular component of mouse fibroblasts. Fibroblasts are the least specialized cells in the connective-tissue family. They are dispersed in connective tissue throughout the body, where they secrete a nonrigid extracellular matrix (ECM) that is rich in type I and/or type III collagen. Conective tissue consists of glycosaminoglycans, proteoglycans and glycoproteins through which various fibres run. These fibres can be collagenous, elastic or reticular. Reticular fibres are composed from the family of collagen proteins and give tensile strength. These fibres are made by reticular fibroblasts. The activation of fibroblasts by inflammatory stimuli results in their migration, proliferation and deposition of extracellular matrix components, important features involved in both wound healing and fibrosis. The ER-TR7 antigen is a ubiquitous component of stromal (interstitial) matrix cartilage and of at least some basement membrane zones. The antigen detected is not a basement membrane component, nor any major collagen type or fibronectin. The antigen detected has a wider tissue distribution than reticulin. ER-TR7 detects an intracellular component of fibroblasts. Since ER-TR7 does not react with purified laminin, collagen types I-V, fibronectin, heparin sulfate proteoglycan, entactin or nidogen, it detects a hitherto uncharacterized antigen. The monoclonal antibody ER-TR7 can be used to study the micro-anatomy of various organs. ER- TR7 outlines the various compartments of peripheral lymphoid organs by characteristic labeling patterns (no such compartments are found in central lymphoid organs). Furthermore ER-TR7 delineates various types of connective tissue compartments in nonlymphoid organs. The antibody ER-TR7 detects reticular fibroblasts, which constitute the cellular framework of lymphoid and nonlymphoid organs and their products. ER-TR7 is useful to clearly delineated the follicles, periarteriolar lymphoid sheath and marginal zone, the major white pulp compartments. Furthermore in lymph nodes, the capsule, sinuses, follicles, paracortex and medullary cords are clearly delineated. Immunogen Mouse thymic stromal cells