RNF8 Protein (AA 1-485) (GST tag)
(2 references)-
- Target See all RNF8 Proteins
- RNF8 (Ring Finger Protein 8 (RNF8))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-485
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This RNF8 protein is labelled with GST tag.
- Application
- ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)
- Purpose
- RNF8 (Human) Recombinant Protein (P01)
- Sequence
- MGEPGFFVTG DRAGGRSWCL RRVGMSAGWL LLEDGCEVTV GRGFGVTYQL VSKICPLMIS RNHCVLKQNP EGQWTIMDNK SLNGVWLNRA RLEPLRVYSI HQGDYIQLGV PLENKENAEY EYEVTEEDWE TIYPCLSPKN DQMIEKNKEL RTKRKFSLDE LAGPGAEGPS NLKSKINKVS CESGQPVKSQ GKGEVASTPS DNLDPKLTAL EPSKTTGAPI YPGFPKVTEV HHEQKASNSS ASQRSLQMFK VTMSRILRLK IQMQEKHEAV MNVKKQTQKG NSKKVVQMEQ ELQDLQSQLC AEQAQQQARV EQLEKTFQEE EQHLQGLEIA QGEKDLKQQL AQALQEHWAL MEELNRSKKD FEAIIQAKNK ELEQTKEEKE KMQAQKEEVL SHMNDVLENE LQCIICSEYF IEAVTLNCAH SFCSYCINEW MKRKIECPIC RKDIKSKTYS LVLDNCINKM VNNLSSEVKE RRIVLIRERK AKRLF
- Characteristics
- Human RNF8 full-length ORF ( AAH07517, 1 a.a. - 485 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Ring Finger Protein 8 (RNF8) (AA 1-485) protein (Strep Tag)
Crystallography grade RNF8 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Ring Finger Protein 8 (RNF8) (AA 1-488) protein (Strep Tag)Crystallography grade RNF8 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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-
: "Solubility-based genetic screen identifies RING finger protein 126 as an E3 ligase for activation-induced cytidine deaminase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 3, pp. 1029-34, (2013) (PubMed).
: "Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1." in: Nature, Vol. 466, Issue 7309, pp. 941-6, (2010) (PubMed).
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: "Solubility-based genetic screen identifies RING finger protein 126 as an E3 ligase for activation-induced cytidine deaminase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 3, pp. 1029-34, (2013) (PubMed).
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- Target
- RNF8 (Ring Finger Protein 8 (RNF8))
- Alternative Name
- RNF8 (RNF8 Products)
- Synonyms
- hRNF8 Protein, 3830404E21Rik Protein, AIP37 Protein, RNF8 Protein, rnf8 Protein, fc66f09 Protein, wu:fc66f09 Protein, zgc:55936 Protein, zgc:77313 Protein, ring finger protein 8 Protein, ring finger protein 8, E3 ubiquitin protein ligase S homeolog Protein, ring finger protein 8, E3 ubiquitin protein ligase L homeolog Protein, ring finger protein 8, E3 ubiquitin protein ligase Protein, RNF8 Protein, Rnf8 Protein, rnf8.S Protein, rnf8.L Protein, rnf8 Protein
- Background
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Full Gene Name: ring finger protein 8
Synonyms: FLJ12013,KIAA0646 - Gene ID
- 9025
- Pathways
- Production of Molecular Mediator of Immune Response
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