Beclin 1 Protein (AA 1-450) (GST tag)
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- Target See all Beclin 1 (BECN1) Proteins
- Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-450
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This Beclin 1 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- BECN1 (Human) Recombinant Protein (P01)
- Sequence
- MEGSKTSNNS TMQVSFVCQR CSQPLKLDTS FKILDRVTIQ ELTAPLLTTA QAKPGETQEE ETNSGEEPFI ETPRQDGVSR RFIPPARMMS TESANSFTLI GEASDGGTME NLSRRLKVTG DLFDIMSGQT DVDHPLCEEC TDTLLDQLDT QLNVTENECQ NYKRCLEILE QMNEDDSEQL QMELKELALE EERLIQELED VEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQQ LELDDELKSV ENQMRYAQTQ LDKLKKTNVF NATFHIWHSG QFGTINNFRL GRLPSVPVEW NEINAAWGQT VLLLHALANK MGLKFQRYRL VPYGNHSYLE SLTDKSKELP LYCSGGLRFF WDNKFDHAMV AFLDCVQQFK EEVEKGETRF CLPYRMDVEK GKIEDTGGSG GSYSIKTQFN SEEQWTKALK FMLTNLKWGL AWVSSQFYNK
- Characteristics
- Human BECN1 full-length ORF ( NP_003757.1, 1 a.a. - 450 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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High Beclin 1 expression defines a poor prognosis in endometrial adenocarcinomas." in: Gynecologic oncology, Vol. 123, Issue 1, pp. 147-51, (2011) (PubMed).
: "Depletion of Beclin-1 due to proteolytic cleavage by caspases in the Alzheimer's disease brain." in: Neurobiology of disease, Vol. 43, Issue 1, pp. 68-78, (2011) (PubMed).
: "Developmentally regulated ceramide synthase 6 increases mitochondrial Ca2+ loading capacity and promotes apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 6, pp. 4644-58, (2011) (PubMed).
: "
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High Beclin 1 expression defines a poor prognosis in endometrial adenocarcinomas." in: Gynecologic oncology, Vol. 123, Issue 1, pp. 147-51, (2011) (PubMed).
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- Target
- Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))
- Alternative Name
- BECN1 (BECN1 Products)
- Background
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Full Gene Name: beclin 1, autophagy related
Synonyms: ATG6,VPS30,beclin1 - Gene ID
- 8678
- NCBI Accession
- NM_003766
- Pathways
- Autophagy
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