Abeta 38/40/42 (AA 1-16), (AA 4-16) antibody

Catalog No. ABIN1742437

Product Details

Target: Abeta 38/40/42

Binding Specificity: AA 1-16, AA 4-16

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Specificity: Specific for Abeta 38, 40, 42.

Purification: purified IgG. Azide was added before lyophilization.

Immunogen: Synthetic peptide (aa 1-16) of abeta peptides 38, 40, 42 coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue. Epitope aa 4-16

Clone: NT78

Isotype: IgG2b

Application Details

Application Notes: WB: 1 : 500 up to 1 : 1000
IP: not tested yet
ICC: not tested yet
IHC-P: 1 : 200 up to 1 : 500

Comment:

ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References

Jawhar, Wirths, Schilling, Graubner, Demuth, Bayer et al.: "Overexpression of glutaminyl cyclase, the enzyme responsible for pyroglutamate A{beta} formation, induces behavioral deficits, and glutaminyl cyclase knock-out rescues the behavioral phenotype in ..." in: The Journal of biological chemistry, Vol. 286, Issue 6, pp. 4454-60, (2011) (PubMed).

Wirths, Erck, Martens, Harmeier, Geumann, Jawhar, Kumar, Multhaup, Walter, Ingelsson, Degerman-Gunnarsson, Kalimo, Huitinga, Lannfelt, Bayer: "Identification of low molecular weight pyroglutamate A{beta} oligomers in Alzheimer disease: a novel tool for therapy and diagnosis." in: The Journal of biological chemistry, Vol. 285, Issue 53, pp. 41517-24, (2010) (PubMed).

Target Details

Target: Abeta 38/40/42