ENO1 antibody (Middle Region)

Catalog No. ABIN2775758

Product Details anti-ENO1 Antibody

Target: ENO1 (Enolase 1 (ENO1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Guinea Pig, Horse, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: VAASEFFRSG KYDLDFKSPD DPSRYISPDQ LADLYKSFIK DYPVVSIEDP

Predicted Reactivity: Cow: 79%, Dog: 93%, Guinea Pig: 93%, Horse: 79%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against ENO1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ENO1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 434 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ENO1 antibody (ABIN2775758)

Braceland, McLoughlin, Tinsley, Wallace, Cockerill, McLaughlin, Eckersall: "Serum enolase: a non-destructive biomarker of white skeletal myopathy during pancreas disease (PD) in Atlantic salmon Salmo salar L." in: Journal of fish diseases, Vol. 38, Issue 9, pp. 821-31, (2015) (PubMed).

Kitahashi, Yoshimoto, Imai: "Novel immunohistochemical marker, integrin ?(V)?(3), for BOP-induced early lesions in hamster pancreatic ductal carcinogenesis." in: Oncology letters, Vol. 2, Issue 2, pp. 229-234, (2012) (PubMed).

Deng, Lam, Meyer, Feldon, Li, Wei, Luk, Chua, Sham, Wang, McAlonan: "Frontal-subcortical protein expression following prenatal exposure to maternal inflammation." in: PLoS ONE, Vol. 6, Issue 2, pp. e16638, (2011) (PubMed).

Sørensen, Horsman, Vorum, Honoré, Overgaard, Alsner: "Proteins upregulated by mild and severe hypoxia in squamous cell carcinomas in vitro identified by proteomics." in: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, Vol. 92, Issue 3, pp. 443-9, (2009) (PubMed).

Boraldi, Annovi, Carraro, Naldini, Tiozzo, Sommer, Quaglino: "Hypoxia influences the cellular cross-talk of human dermal fibroblasts. A proteomic approach." in: Biochimica et biophysica acta, Vol. 1774, Issue 11, pp. 1402-13, (2007) (PubMed).

Target Details for ENO1

Target: ENO1 (Enolase 1 (ENO1))

Alternative Name: ENO1 (ENO1 Products)

Background: ENO1 encodes one of three enolase isoenzymes found in mammals, it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.
Alias Symbols: NNE, PPH, MPB1, ENO1L1
Protein Interaction Partner: UBC, FUS, HUWE1, ISG15, SUMO2, SUMO3, IVNS1ABP, STAU1, MDM2, WWOX, EED, FBXO6, UPF2, YWHAQ, UBD, TERT, SRC, RAD52, NPM1, BHLHE40, VCAM1, HCVgp1, ITGA4, FN1, ATF2, SERPING1, AMBP, LIG4, BRCA1, MDC1, PGK1, ITPA, GAPDH, FYN, FLNC, ENO3, Htt, TPI1, PPIA, AKT1
Protein Size: 434

Molecular Weight: 47 kDa

Gene ID: 2023

NCBI Accession: NM_001428, NP_001419

UniProt: Q53HR3