GCM1 antibody (N-Term)

Catalog No. ABIN2792608

Product Details anti-GCM1 Antibody

Target: GCM1 (Glial Cells Missing Homolog 1 (GCM1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: MEPDDFDSED KEILSWDIND VKLPQNVKKT DWFQEWPDSY AKHIYSSEDK

Cross-Reactivity: Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Dog: 78%, Human: 92%, Mouse: 78%, Rat: 78%

Characteristics: This is a rabbit polyclonal antibody against GCM1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GCM1

Application Details

Application Notes: WB Suggested Anti-GCM1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:7812500
Positive Control: Human Placenta.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 436 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GCM1 antibody (ABIN2792608)

Bailey, Alahari, Tagliaferro, Post, Caniggia: "Augmented trophoblast cell death in preeclampsia can proceed via ceramide-mediated necroptosis." in: Cell death & disease, Vol. 8, Issue 2, pp. e2590, (2017) (PubMed).

Moretto Zita, Soncin, Natale, Pizzo, Parast: "Gene Expression Profiling Reveals a Novel Regulatory Role for Sox21 Protein in Mouse Trophoblast Stem Cell Differentiation." in: The Journal of biological chemistry, Vol. 290, Issue 50, pp. 30152-62, (2015) (PubMed).

Sivasubramaniyam, Garcia, Tagliaferro, Melland-Smith, Chauvin, Post, Todros, Caniggia: "Where polarity meets fusion: role of Par6 in trophoblast differentiation during placental development and preeclampsia." in: Endocrinology, Vol. 154, Issue 3, pp. 1296-309, (2013) (PubMed).

Kumar, Luo, Tudela, Alexander, Mendelson: "The c-Myc-regulated microRNA-17~92 (miR-17~92) and miR-106a~363 clusters target hCYP19A1 and hGCM1 to inhibit human trophoblast differentiation." in: Molecular and cellular biology, Vol. 33, Issue 9, pp. 1782-96, (2013) (PubMed).

Drewlo, Czikk, Baczyk, Lye, Kingdom: "Glial cell missing-1 mediates over-expression of tissue inhibitor of metalloproteinase-4 in severe pre-eclamptic placental villi." in: Human reproduction (Oxford, England), Vol. 26, Issue 5, pp. 1025-34, (2011) (PubMed).

Baczyk, Drewlo, Proctor, Dunk, Lye, Kingdom: "Glial cell missing-1 transcription factor is required for the differentiation of the human trophoblast." in: Cell death and differentiation, Vol. 16, Issue 5, pp. 719-27, (2009) (PubMed).

Target Details for GCM1

Target: GCM1 (Glial Cells Missing Homolog 1 (GCM1))

Alternative Name: GCM1 / GCMA (GCM1 Products)

Synonyms: GCM1 antibody, GCM antibody, GCM-1 antibody, GCMA antibody, hGCMa antibody, GCMa antibody, Gcm-rs2 antibody, Gcm1-rs1 antibody, glide antibody, Gcma antibody, glial cells missing homolog 1 antibody, glial cells missing homolog 1 (Drosophila) antibody, GCM1 antibody, Gcm1 antibody

Background: GCM1 is a DNA-binding protein with a gcm-motif (glial cell missing motif). The encoded protein is a homolog of the Drosophila glial cells missing gene (gcm). This protein binds to the GCM-motif (A/G)CCCGCAT, a novel sequence among known targets of DNA-binding proteins. The N-terminal DNA-binding domain confers the unique DNA-binding activity of this protein.This gene encodes a DNA-binding protein with a gcm-motif (glial cell missing motif). The encoded protein is a homolog of the Drosophila glial cells missing gene (gcm). This protein binds to the GCM-motif (A/G)CCCGCAT, a novel sequence among known targets of DNA-binding proteins. The N-terminal DNA-binding domain confers the unique DNA-binding activity of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-132 D88613.1 1-132 133-2763 AB026493.1 1-2631
Alias Symbols: GCMA, hGCMa
Protein Interaction Partner: FBXW2, UBC, SENP1, CAMK1, SUMO1, DUSP23, CREBBP, HDAC5, HDAC4, HDAC3, HDAC1, CUL1, SKP1, UBE2I,
Protein Size: 436

Molecular Weight: 49 kDa

Gene ID: 8521

NCBI Accession: NM_003643, NP_003634

UniProt: Q9NP62