DBN1 antibody (AA 1-326) (PE)

Catalog No. ABIN1558085

Product Details anti-DBN1 Antibody (PE)

Target: DBN1 (Drebrin 1 (DBN1))

Binding Specificity: AA 1-326

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DBN1 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody DBN-N-03 recognizes drebrin, an approximately 100-125 kDa intracellular regulator of actin cytoskeleton.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Bacterially expressed N-terminal fragment of recombinant human drebrin (aa 1-326)

Clone: DBN-N-03

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-DBN1 antibody (PE) (ABIN1558085)

Vaskova, Kovac, Volna, Angelisova, Mejstrikova, Zuna, Brdicka, Hrusak: "High expression of cytoskeletal protein drebrin in TEL/AML1pos B-cell precursor acute lymphoblastic leukemia identified by a novel monoclonal antibody." in: Leukemia research, Vol. 35, Issue 8, pp. 1111-3, (2011) (PubMed).

Target Details for DBN1

Target: DBN1 (Drebrin 1 (DBN1))

Alternative Name: Drebrin (DBN1 Products)

Synonyms: D0S117E antibody, DBN1 antibody, LOC733967 antibody, drebrin 1 antibody, drebrin 1 S homeolog antibody, DBN1 antibody, Dbn1 antibody, dbn1.S antibody, dbn1 antibody

Background: Drebrin 1,Drebrin is an actin-binding protein, which is expressed mainly in neurons and plays important role in their morphogenesis. The highest level of its expression is in developing brain. Both in neurons and non-neuronal cells drebrin acts as a key regulator of actin cytoskeleton remodelling, affecting especially intercellular junctions, such as dendritic spines of neurons or the immune synapses of T cells. Decrease of drebrin amount in the brain seems to be associated with Alzheimer's disease and Down syndrome, and in case of B-cell precursor acute lymphoblastic leukemia (BCP-ALL) lower drebrin expression correlates with higher risk of relapse.,DBN, D0S117E

Gene ID: 1627

UniProt: Q16643

Pathways: Maintenance of Protein Location