SensoLyte® 520 MMP-2 Assay Kit

Catalog No. ABIN1882519

Product Details

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: SensoLyte® 520 MMP-2 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-2, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Components A and B from light.

Storage: -20 °C

Storage Comment: Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.

References

Liu, Wu, Zhou, Peng: "Endothelin A receptor antagonism enhances inhibitory effects of anti-ganglioside GD2 monoclonal antibody on invasiveness and viability of human osteosarcoma cells." in: PLoS ONE, Vol. 9, Issue 4, pp. e93576, (2014) (PubMed).

Pifer, Maerz, Baker, Anderson et al.: "Matrix metalloproteinase content and activity in low-platelet, low-leukocyte and high-platelet, high-leukocyte platelet rich plasma (PRP) and the biologic response to PRP by human ligament ..." in: The American journal of sports medicine, Vol. 42, Issue 5, pp. 1211-8, (2014) (PubMed).

Spolidoro, Putignano, Munafò, Maffei, Pizzorusso: "Inhibition of matrix metalloproteinases prevents the potentiation of nondeprived-eye responses after monocular deprivation in juvenile rats." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 22, Issue 3, pp. 725-34, (2012) (PubMed).

Jo, Park, Shin, Kim, Hwang, Cho, Kim: "ARP101, a selective MMP-2 inhibitor, induces autophagy-associated cell death in cancer cells." in: Biochemical and biophysical research communications, Vol. 404, Issue 4, pp. 1039-43, (2011) (PubMed).

Qazi, Shi, Tarbell: "Fluid shear stress regulates the invasive potential of glioma cells via modulation of migratory activity and matrix metalloproteinase expression." in: PLoS ONE, Vol. 6, Issue 5, pp. e20348, (2011) (PubMed).

Yuan, Johnson, Chen, Wei, Pellecchia: "A novel pharmacophore model for the design of anthrax lethal factor inhibitors." in: Chemical biology & drug design, Vol. 76, Issue 3, pp. 263-8, (2010) (PubMed).

Rahn, Acevedo, Roshanravan, Keller, Davis, Marmorstein, Word: "Failure of pelvic organ support in mice deficient in fibulin-3." in: The American journal of pathology, Vol. 174, Issue 1, pp. 206-15, (2009) (PubMed).

Shi, Ji, Qazi, Tarbell: "Interstitial flow promotes vascular fibroblast, myofibroblast, and smooth muscle cell motility in 3-D collagen I via upregulation of MMP-1." in: American journal of physiology. Heart and circulatory physiology, Vol. 297, Issue 4, pp. H1225-34, (2009) (PubMed).

Wieslander, Rahn, McIntire, Acevedo, Drewes, Yanagisawa, Word: "Quantification of pelvic organ prolapse in mice: vaginal protease activity precedes increased MOPQ scores in fibulin 5 knockout mice." in: Biology of reproduction, Vol. 80, Issue 3, pp. 407-14, (2009) (PubMed).

Wieslander, Marinis, Drewes, Keller, Acevedo, Word: "Regulation of elastolytic proteases in the mouse vagina during pregnancy, parturition, and puerperium." in: Biology of reproduction, Vol. 78, Issue 3, pp. 521-8, (2008) (PubMed).

Target Details

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Alternative Name: MMP-2 (MMP2 Products)

Synonyms: 2-MMP Kit, CG1794 Kit, DM2-MMP Kit, Dm2-MMP Kit, Dmel\\CG1794 Kit, MMP2 Kit, anon-WO0118547.84 Kit, dm-2MMP Kit, dmmp2 Kit, l(2)02353 Kit, mmp2 Kit, wu:fa99h12 Kit, wu:fk89d01 Kit, fgmmp-2 Kit, Mmp-2 Kit, LOC100135793 Kit, Clg4a Kit, GelA Kit, MMP-2 Kit, CLG4 Kit, CLG4A Kit, MMP-II Kit, MONA Kit, TBE-1 Kit, matrix metallopeptidase 2 Kit, Matrix metalloproteinase 2 Kit, matrix metalloproteinase 2 Kit, matrix metalloproteinase-2 Kit, MMP2 Kit, Mmp2 Kit, mmp2 Kit, LOC657982 Kit, LOC100135793 Kit

Background: Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion is well known. MMP-2 (gelatinase A) is proposed as a potential therapeutic target.