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ENO1 Protein (AA 1-434) (GST tag)

Name: ENO1 Protein (AA 1-434) (GST tag)
SKU: ABIN1352797
Price: 510.67 USD
Availability: InStock

ENO1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP

Image no. 1 for Enolase 1 (ENO1) (AA 1-434) protein (GST tag) (ABIN1352797)

1 image

(4 references)

Catalog No. ABIN1352797

Datasheet as PDF

Target See all ENO1 Proteins

ENO1 (Enolase 1 (ENO1))

Protein Type

Recombinant

Protein Characteristics

AA 1-434
Origin
11

6

3

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Human
Source
18

17

3

1

1
Wheat germ

Purification tag / Conjugate

This ENO1 protein is labelled with GST tag.

Application

Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Purpose

ENO1 (Human) Recombinant Protein (P01)

Sequence

MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEGLGSK AKFAGRNFRN PLAK

Characteristics

Human ENO1 full-length ORF ( AAH11130.1, 1 a.a. - 434 a.a.) recombinant protein with GST-tag at N-terminal.

Purification

in vitro wheat germ expression system
Enolase 1 (ENO1) (AA 325-434) protein (GST tag)
ENO1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP

Enolase 1 (ENO1) (AA 1-434) protein
ENO1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS

Enolase 1 (ENO1) (AA 2-433) protein (His tag)
ENO1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA

Enolase 1 (ENO1) protein (His tag)
ENO1 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by reducing SDS-PAGE.

Enolase 1 (ENO1) (AA 2-434) protein (His tag)
Crystallography grade ENO1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys

Enolase 1 (ENO1) (AA 2-434) protein (His tag)
Crystallography grade ENO1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys

Enolase 1 (ENO1) (AA 2-428), (partial) protein (GST tag)
ENO1 Host: Escherichia coli (E. coli) Recombinant 90 % ELISA

Enolase 1 (ENO1) (AA 2-433), (partial) protein (His tag)
ENO1 Origin: Mouse Host: Yeast Recombinant > 90 % ELISA

Enolase 1 (ENO1) protein (GST tag)
ENO1 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by reducing SDS-PAGE.

Enolase 1 (ENO1) (Active) protein (His tag)
ENO1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant SDS Active

Application Notes

Optimal working dilution should be determined by the investigator.

Comment

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions

For Research Use only
Buffer

50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice

Aliquot to avoid repeated freezing and thawing.

Storage

-80 °C

Storage Comment

Best use within three months from the date of receipt of this protein.
Bruschi, Carnevali, Murtas, Candiano, Petretto, Prunotto, Gatti, Argentiero, Magistroni, Garibotto, Scolari, Ravani, Gesualdo, Allegri, Ghiggeri: "Direct characterization of target podocyte antigens and auto-antibodies in human membranous glomerulonephritis: Alfa-enolase and borderline antigens." in: Journal of proteomics, Vol. 74, Issue 10, pp. 2008-17, (2011) (PubMed).

Terrier, Tamby, Camoin, Guilpain, Bérezné, Tamas, Broussard, Hotellier, Humbert, Simonneau, Guillevin, Mouthon: "Antifibroblast antibodies from systemic sclerosis patients bind to {alpha}-enolase and are associated with interstitial lung disease." in: Annals of the rheumatic diseases, Vol. 69, Issue 2, pp. 428-33, (2010) (PubMed).

Lu, Jia, He, Hurley, Leys, Jayasundera, Heckenlively: "Melanoma-associated retinopathy: a paraneoplastic autoimmune complication." in: Archives of ophthalmology, Vol. 127, Issue 12, pp. 1572-80, (2009) (PubMed).

Vermeulen, Arijs, Joossens, Vermeire, Clerens, Van den Bergh, Michiels, Arckens, Schuit, Van Lommel, Rutgeerts, Bossuyt: "Anti-alpha-enolase antibodies in patients with inflammatory Bowel disease." in: Clinical chemistry, Vol. 54, Issue 3, pp. 534-41, (2008) (PubMed).
Target

ENO1 (Enolase 1 (ENO1))

Alternative Name

ENO1 (ENO1 Products)

Synonyms

enolase 1 Protein, F2P9.10 Protein, F2P9_10 Protein, Pgh1 Protein, pENO1 Protein, enolase 1 Protein, phosphopyruvate hydratase Protein, ENO1 Protein, eno1 Protein

Background

Full Gene Name: enolase 1, (alpha)
Synonyms: ENO1L1,MBP-1,MPB1,NNE,PPH

Gene ID

2023