Recombinant TNFSF13 antibody (AA 98-232)

Catalog No. ABIN1169000

Product Details anti-TNFSF13 Antibody

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Antibody Type: Recombinant Antibody

Binding Specificity: AA 98-232

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFSF13 antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP), Functional Studies (Func)

Specificity: Recognizes mouse APRIL.

Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): Does not recognize mouse BAFF.

Purification: Purified from HEK 293 cell culture supernatant.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.01EU/μg purified protein (LAL-test, Lonza)

Immunogen: Mouse APRIL (aa 98-232).

Clone: Apry-1-1

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

APRIL (mouse) monoclonal antibody (recombinant) (blocking) (APRY-1-1) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the mouse lgG2b Fc domain.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-TNFSF13 antibody (ABIN1169000)

Matthes, Dunand-Sauthier, Santiago-Raber, Krause, Donze, Passweg, McKee, Huard: "Production of the plasma-cell survival factor a proliferation-inducing ligand (APRIL) peaks in myeloid precursor cells from human bone marrow." in: Blood, Vol. 118, Issue 7, pp. 1838-44, (2011) (PubMed).

Target Details for TNFSF13

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Alternative Name: APRIL (TNFSF13 Products)

Synonyms: APRIL antibody, CD256 antibody, TALL-2 antibody, TALL2 antibody, TRDL-1 antibody, ZTNF2 antibody, 2310026N09Rik antibody, April antibody, Tall2 antibody, Trdl1 antibody, SLAN antibody, ANP32B antibody, april antibody, tnf13 antibody, TNLG7B antibody, TNF superfamily member 13 antibody, tumor necrosis factor (ligand) superfamily, member 13 antibody, acidic leucine-rich nuclear phosphoprotein 32 family member B antibody, TNFSF13 antibody, Tnfsf13 antibody, LOC494442 antibody, tnfsf13 antibody

Background: APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth and is involved in monocyte/macrophage-mediated immunological processes. Anti-APRIL (mouse) Monoclonal Antibody (recombinant) (Blocking) (APRY-1-1) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

UniProt: Q9D777

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response