Rabbit IgG Isotype Control

Catalog No. ABIN398653

Product Details

Target: IgG

Host: Rabbit

Application: Isotype Control (IsoC), ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunoelectrophoresis (IEP), Dot Blot (DB), Immunodiffusion (ID), Blocking Reagent (BR)

Characteristics: Purified Rabbit IgG (Whole Molecule) Control is highly purified from normal rabbit serum by Protein G chromatography.Purified Rabbit IgG (Whole Molecule) Control is suitable for use as control, standard, blocking agent, or coating protein in a variety of assays, including ELISA, immunobloting (Dot blot and Western blot), immunoprecipitation, immunodiffusion, and immunoelectrophoresis. It also may be used as antigen or ligand in immunochemical reactions and conjugations.

Purification: Protein G chromatography

Isotype: IgG

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 4 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.

References

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Shan, Xu, Bleyer, Becker, Melbaum, Wemheuer, Hirschfeld, Wacker, Zhao, Schütz, Brenig: "Association of α/β-Hydrolase D16B with Bovine Conception Rate and Sperm Plasma Membrane Lipid Composition." in: International journal of molecular sciences, Vol. 21, Issue 2, (2020) (PubMed).

Aeckerle, Drummer, Debowski, Viebahn, Behr: "Primordial germ cell development in the marmoset monkey as revealed by pluripotency factor expression: suggestion of a novel model of embryonic germ cell translocation." in: Molecular human reproduction, Vol. 21, Issue 1, pp. 66-80, (2015) (PubMed).

Timblin, Schlissel: "Ebf1 and c-Myb repress rag transcription downstream of Stat5 during early B cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 9, pp. 4676-87, (2013) (PubMed).

Cowan, Singh, Ghert: "PTHrP increases RANKL expression by stromal cells from giant cell tumor of bone." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 30, Issue 6, pp. 877-84, (2012) (PubMed).

Cowan, Ghert, Singh: "T cells stimulate catabolic gene expression by the stromal cells from giant cell tumor of bone." in: Biochemical and biophysical research communications, Vol. 419, Issue 4, pp. 719-23, (2012) (PubMed).

Gee, Milgram, Kramer, Conlon, Moody: "Yes-associated protein 65 (YAP) expands neural progenitors and regulates Pax3 expression in the neural plate border zone." in: PLoS ONE, Vol. 6, Issue 6, pp. e20309, (2011) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody