UGP2 antibody

Catalog No. ABIN2559390

Product Details anti-UGP2 Antibody

Target: UGP2 (UDP-Glucose Pyrophosphorylase 2 (UGP2))

Reactivity: Barley, Tomato, Solanum sogarandinum, Potato, Oryza sativa, Nicotiana tabacum, Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UGP2 antibody is un-conjugated

Application: Western Blotting (WB)

Characteristics: Expected / apparent Molecular Weight of the Antigene: 51.6 kDa

Purification: serum

Immunogen: recombinant UGPase Q43772 overexpressed and purified from E.coli

Application Details

Application Notes: Recommended Dilution: 1 : 1000 - 1 : 3000 with standard ECL (WB).
Cellular [compartment marker] of cytoplasm

Comment:

this antibody detectes 1 ng of UGPase in a western blot and reacts with both cytosolic isoforms only which have similar MW of ca. 52 kDa in Arabidopsis thaliana

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-UGP2 antibody (ABIN2559390)

McLoughlin, Arisz, Dekker, Kramer, de Koster, Haring, Munnik, Testerink: "Identification of novel candidate phosphatidic acid-binding proteins involved in the salt-stress response of Arabidopsis thaliana roots." in: The Biochemical journal, Vol. 450, Issue 3, pp. 573-81, (2013) (PubMed).

Yasui, Mukougawa, Uemoto, Yokofuji, Suzuri, Nishitani, Kohchi: "The phytochrome-interacting vascular plant one-zinc finger1 and VOZ2 redundantly regulate flowering in Arabidopsis." in: The Plant cell, Vol. 24, Issue 8, pp. 3248-63, (2012) (PubMed).

Foreman, Johansson, Hornitschek, Josse, Fankhauser, Halliday: "Light receptor action is critical for maintaining plant biomass at warm ambient temperatures." in: The Plant journal : for cell and molecular biology, Vol. 65, Issue 3, pp. 441-52, (2011) (PubMed).

Balsemão-Pires, Jaillais, Olson, Andrade, Umen, Chory, Sachetto-Martins: "The Arabidopsis translocator protein (AtTSPO) is regulated at multiple levels in response to salt stress and perturbations in tetrapyrrole metabolism." in: BMC plant biology, Vol. 11, pp. 108, (2011) (PubMed).

Sidaway-Lee, Josse, Brown, Gan, Halliday, Graham, Penfield: "SPATULA links daytime temperature and plant growth rate." in: Current biology : CB, Vol. 20, Issue 16, pp. 1493-7, (2010) (PubMed).

Xiong, Li, Qian, Song, Liu, Yu, Zeng, Wan, Li, Zhou: "The rice dynamin-related protein DRP2B mediates membrane trafficking and thereby plays a critical role in secondary cell wall cellulose biosynthesis." in: The Plant journal : for cell and molecular biology, (2010) (PubMed).

Ito, Batth, Petzold, Redding-Johanson, Mukhopadhyay, Verboom, Meyer, Millar, Heazlewood: "Analysis of the Arabidopsis Cytosolic Proteome Highlights Subcellular Partitioning of Central Plant Metabolism." in: Journal of proteome research, (2010) (PubMed).

Meng, Wilczynska, Kleczkowski: "Molecular and kinetic characterization of two UDP-glucose pyrophosphorylases, products of distinct genes, from Arabidopsis." in: Biochimica et biophysica acta, Vol. 1784, Issue 6, pp. 967-72, (2008) (PubMed).

Pujol, Bailly, Kern, Marechal-Drouard, Becker, Duchene: "Dual-targeted tRNA-dependent amidotransferase ensures both mitochondrial and chloroplastic Gln-tRNAGln synthesis in plants." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 17, pp. 6481-5, (2008) (PubMed).

Target Details for UGP2

Target: UGP2 (UDP-Glucose Pyrophosphorylase 2 (UGP2))

Alternative Name: UGPase (UGP2 Products)

Background: AGI Code: At3g03250
UDP-glucose pyrophosphorylase (UGPase, UDPGP) E.C=2.7.7.9. is a key enzyme of synthesis of sucrose, cellulose and other saccharides. There are two cytoplasmic isoforms of UGPase-A (which share 94 % identity on amino acid level) and one chloroplastic UGPase-B isoform in Arabidopsis thaliana which share ca. 10-11 % of identity (Kleczkowski et al. 2011).

Molecular Weight: 51.6 kDa

UniProt: Q43772

Pathways: Cellular Glucan Metabolic Process