Fibroblasts (Reticular) antibody

Catalog No. ABIN111871

Product Details

Target: Fibroblasts (Reticular)

Reactivity: Mouse, Human, Pig

Host: Rat

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Reacts with Mouse reticular fibroblasts, reticular fibres and Human tonsil. ER-TR7 detects an antigen present in and produced by reticular fibroblasts. The recognized antigen is most likely distinct from Laminin, Fibronectin, Collagen types I-IV, Heparan Sulfate Proteoglycan, Entactin, and Nidogen. The antibody is useful to stain the microanatomy of various organs, in particular the connective tissue framework in lymphoid organs. The antibody also stains subendothelial deposits in the plaque area of atherosclerotic plaques. Antigen Distribution Isolated Cells: The antigen is found in the cytoplasm of fibroblast cell lines. Tissue Sections: The antigen is expressed in connective tissues which form a supporting network between parenchymal cells of all organs tested so far (see table below). Thus, the supportive mesenchymal structures of larger vessels can be studied. In spleen, a very clear delineation of red and white pulp is obtained. Capsule, sinuses, follicules, paracortex and medullary cords are also clearly delineated in lymph nodes.Antigen Distribution

Cross-Reactivity (Details): Species reactivity (tested):Mouse (Reticular Fibroblasts, Reticular Fibres), Pig and Human.

Purification: Affinity Chromatography

Immunogen: Murine thymic reticulum. Epitope: The antigen has not been fully characterized. The epitope may be part of reticulin.

Clone: ER-TR7

Isotype: IgG2a

Application Details

Application Notes: Immunofluorescence. Immunohistochemistry on Frozen Sections: 1 μg/mL (1/400) freshly prepared for Mousetissue and 4 μg/mL (1/100) on Human/Porcine tissue. Recommended Positive Control: Mouse spleen. Does not react on routinely processed Paraffin Sections. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: (The whole procedure is performed at room temperature)1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L)minimal-cross reaction to mouse) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayers hemalum

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 4-8 °C for one month or at -20 °C for longer.

References

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Target Details

Target: Fibroblasts (Reticular)

Background: Connective tissue consists of a ground of glycosaminoglycans, proteoglycans and glycoproteins through which various fibres run. These fibres can be collagenous, elastic or reticular. Reticular fibres are composed from the family of collagen proteins and give tensile strength. These fibres are made by reticular fibroblasts.Synonyms: Fibroblast Marker, Fibroblasten