SNRNP70 antibody (C-Term)

Catalog No. ABIN2778829

Product Details anti-SNRNP70 Antibody

Target: SNRNP70 (Small Nuclear Ribonucleoprotein 70kDa (U1) (SNRNP70))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNRNP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: RERERKEELR GGGGDMAEPS EAGDAPPDDG PPGELGPDGP DGPEEKGRDR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SNRP70. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SNRP70

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 437 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SNRNP70 antibody (ABIN2778829)

Franklin, Chen, Mitchell-Jordan, Ren, Wang, Vondriska: "Quantitative analysis of the chromatin proteome in disease reveals remodeling principles and identifies high mobility group protein B2 as a regulator of hypertrophic growth." in: Molecular & cellular proteomics : MCP, Vol. 11, Issue 6, pp. M111.014258, (2012) (PubMed).

Serini, Fasano, Piccioni, Monego, Cittadini, Celleno, Ranelletti, Calviello: "DHA induces apoptosis and differentiation in human melanoma cells in vitro: involvement of HuR-mediated COX-2 mRNA stabilization and ?-catenin nuclear translocation." in: Carcinogenesis, Vol. 33, Issue 1, pp. 164-73, (2011) (PubMed).

Franklin, Zhang, Chen, Paulsson, Mitchell-Jordan, Li, Ping, Vondriska: "Specialized compartments of cardiac nuclei exhibit distinct proteomic anatomy." in: Molecular & cellular proteomics : MCP, Vol. 10, Issue 1, pp. M110.000703, (2011) (PubMed).

Target Details for SNRNP70

Target: SNRNP70 (Small Nuclear Ribonucleoprotein 70kDa (U1) (SNRNP70))

Alternative Name: SNRP70 (SNRNP70 Products)

Background: SNRP70 contains 1 RRM (RNA recognition motif) domain and mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.
Alias Symbols: RNPU1Z, RPU1, U170K, U1AP, U1RNP, Snp1, SNRP70, U1-70K
Protein Interaction Partner: IL32, UBC, NEDD8, LIN28A, EZH2, SUZ12, EED, RNF2, rev, WBP4, SRPK2, SRPK1, MYC, SRPK3, HDAC2, PAN2, SMURF2, PRPF40A, CDKL3, RNF11, PRKD2, SKIL, RELA, RASGRF1, SMAD9, SMAD5, SMAD4, SMAD3, SMAD2, CHERP, PRMT5, UBL4A, VCAM1, SMAD1, ITGA4, IL7R, IFIT3, IFIT1,
Protein Size: 437

Molecular Weight: 51 kDa

Gene ID: 6625

NCBI Accession: NM_003089, NP_003080

UniProt: P08621