HMGB1 Protein (AA 1-215) (GST tag)

Catalog No. ABIN1306797

Product Details

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Protein Type: Recombinant

Protein Characteristics: AA 1-215

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This HMGB1 protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Purpose: HMGB1 (Human) Recombinant Protein (P01)

Sequence: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDE

Characteristics: Human HMGB1 full-length ORF ( AAH03378.1, 1 a.a. - 215 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Yang, Lee, Song, Bae: "Ameliorative effect of a rarely occurring C-methylrotenoid on HMGB1-induced septic responses in vitro and in vivo." in: Biochemical pharmacology, Vol. 110-111, pp. 58-70, (2016) (PubMed).

Fang, Jiang: "IL-1β/HMGB1 signalling promotes the inflammatory cytokines release via TLR signalling in human intervertebral disc cells." in: Bioscience reports, Vol. 36, Issue 5, (2016) (PubMed).

Min, Ku, Park, Park, Lee, Bae: "Anti-septic effects of pelargonidin on HMGB1-induced responses in vitro and in vivo." in: Archives of pharmacal research, Vol. 39, Issue 12, pp. 1726-1738, (2016) (PubMed).

Lee, Park, Kwak, Kim, Na, Bae: "PEGylated lysozymes with anti-septic effects in human endothelial cells and in mice." in: Biochemical and biophysical research communications, Vol. 459, Issue 4, pp. 662-7, (2015) (PubMed).

Kwak, Ku, Kang, Baek, Bae: "Methylthiouracil, a new treatment option for sepsis." in: Vascular pharmacology, (2015) (PubMed).

Tuan, Lee, Oh, Kulkarni, Gény, Jung, Kang, Bae, Na: "Flavanones and Chromones from Salicornia herbacea Mitigate Septic Lethality via Restoration of Vascular Barrier Integrity." in: Journal of agricultural and food chemistry, Vol. 63, Issue 46, pp. 10121-30, (2015) (PubMed).

Lee, Ku, Bae: "Factor Xa inhibits HMGB1-induced septic responses in human umbilical vein endothelial cells and in mice." in: Thrombosis and haemostasis, Vol. 112, Issue 4, pp. 757-69, (2014) (PubMed).

Ku, Lee, Kim, Bae: "Anti-septic effects of pellitorine in HMGB1-induced inflammatory responses in vitro and in vivo." in: Inflammation, Vol. 37, Issue 2, pp. 338-48, (2014) (PubMed).

Ku, Kim, Bae: "Vascular barrier protective effects of piperlonguminine in vitro and in vivo." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 63, Issue 5, pp. 369-79, (2014) (PubMed).

Lee, Ku, Yoo, Song, Bae: "Andrographolide inhibits HMGB1-induced inflammatory responses in human umbilical vein endothelial cells and in murine polymicrobial sepsis." in: Acta physiologica (Oxford, England), Vol. 211, Issue 1, pp. 176-87, (2014) (PubMed).

Klawitter, Hakozaki, Kobayashi, Krupkova, Quero, Ospelt, Gay, Hausmann, Liebscher, Meier, Sekiguchi, Konno, Boos, Ferguson, Wuertz: "Expression and regulation of toll-like receptors (TLRs) in human intervertebral disc cells." in: European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, Vol. 23, Issue 9, pp. 1878-91, (2014) (PubMed).

Kwak, Ku, Han, Bae: "Vascular barrier protective effects of baicalin, baicalein and wogonin in vitro and in vivo." in: Toxicology and applied pharmacology, Vol. 281, Issue 1, pp. 30-38, (2014) (PubMed).

Yang, Ku, Lee, Lee, Lee, Song, Bae: "Barrier protective effects of rosmarinic acid on HMGB1-induced inflammatory responses in vitro and in vivo." in: Journal of cellular physiology, Vol. 228, Issue 5, pp. 975-82, (2013) (PubMed).

Lee, Ku, Kim, Bae: "Emodin-6-O-?-D-glucoside inhibits HMGB1-induced inflammatory responses in vitro and in vivo." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 52, pp. 97-104, (2013) (PubMed).

Lee, Ku, Kim, Lee, Bae: "Inhibitory effects of epi-sesamin on HMGB1-induced vascular barrier disruptive responses in vitro and in vivo." in: Toxicology and applied pharmacology, Vol. 267, Issue 3, pp. 201-8, (2013) (PubMed).

Lee, Ku, Lee, Bae: "Anti-septic effects of glyceollins in HMGB1-induced inflammatory responses in vitro and in vivo." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 63, pp. 1-8, (2013) (PubMed).

Bae: "Effects of lower concentration thrombin on high-mobility group box 1 protein-mediated inflammatory responses." in: Inflammation, Vol. 35, Issue 3, pp. 1078-86, (2012) (PubMed).

Kim, Ku, Bae: "Inhibitory effects of kaempferol-3-O-sophoroside on HMGB1-mediated proinflammatory responses." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 50, Issue 3-4, pp. 1118-23, (2012) (PubMed).

Yang, Lee, Ku, Song, Bae: "Anti-inflammatory activities of oleanolic acid on HMGB1 activated HUVECs." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 50, Issue 5, pp. 1288-94, (2012) (PubMed).

Lee, Ku, Bae, Bae: "Inhibitory effects of lycopene on HMGB1-mediated pro-inflammatory responses in both cellular and animal models." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 50, Issue 6, pp. 1826-33, (2012) (PubMed).

Target Details

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1 (HMGB1 Products)

Synonyms: HMG1 Protein, HMG3 Protein, SBP-1 Protein, DEF Protein, HMG-1 Protein, Hmg1 Protein, amphoterin Protein, p30 Protein, hmgb1 Protein, ik:tdsubc_1a5 Protein, wu:fb23c02 Protein, xx:tdsubc_1a5 Protein, zgc:56110 Protein, zgc:77104 Protein, hmg-1 Protein, hmg3 Protein, sbp-1 Protein, hmg1 Protein, HMGB1 Protein, Ac2-008 Protein, high mobility group box 1 Protein, high-mobility group box 1 Protein, high mobility group box 1a Protein, high mobility group box 1 L homeolog Protein, high mobility group protein B1 Protein, HMGB1 Protein, Hmgb1 Protein, hmgb1 Protein, hmgb1a Protein, hmgb1.L Protein, LOC100359149 Protein

Background: Full Gene Name: high-mobility group box 1
Synonyms: DKFZp686A04236,HMG1,HMG3,SBP-1

Gene ID: 3146

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome