PKM1 (AA 383-417), (C-Term) antibody

Catalog No. ABIN391688

Product Details

Target: PKM1

Binding Specificity: AA 383-417, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PKM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383-417 amino acids from the C-terminal region of human PKM1.

Clone: RB18404

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References

De Rosa, Iommelli, Monti, Fonti, Votta, Stoppelli, Del Vecchio: "Reversal of Warburg Effect and Reactivation of Oxidative Phosphorylation by Differential Inhibition of EGFR Signaling Pathways in Non-Small Cell Lung Cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 21, Issue 22, pp. 5110-20, (2015) (PubMed).

Hamabe, Konno, Tanuma, Shima, Tsunekuni, Kawamoto, Nishida, Koseki, Mimori, Gotoh, Yamamoto, Doki, Mori, Ishii: "Role of pyruvate kinase M2 in transcriptional regulation leading to epithelial-mesenchymal transition." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 43, pp. 15526-31, (2014) (PubMed).

Xie, Zhou, Jiang, Dai, Puscheck, Lee, Parker, Hüttemann, Rappolee: "Hypoxic stress induces, but cannot sustain trophoblast stem cell differentiation to labyrinthine placenta due to mitochondrial insufficiency." in: Stem cell research, Vol. 13, Issue 3 Pt A, pp. 478-91, (2014) (PubMed).

Ye, Mancuso, Tong, Ward, Fan, Rabinowitz, Thompson: "Pyruvate kinase M2 promotes de novo serine synthesis to sustain mTORC1 activity and cell proliferation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 18, pp. 6904-9, (2012) (PubMed).

Wittwer, Robbins, Wang, Codarin, Shen, Kevil, Huang, Van Remmen, Richardson, Zhao: "Enhancing mitochondrial respiration suppresses tumor promoter TPA-induced PKM2 expression and cell transformation in skin epidermal JB6 cells." in: Cancer prevention research (Philadelphia, Pa.), Vol. 4, Issue 9, pp. 1476-84, (2011) (PubMed).

Sun, Chen, Ma, Peng, Wang, Zha, Wang, Jing, Yang, Chen, Chang, Zhang, Goto, Onda, Chen, Wang, Lu, You, Kwiatkowski, Zhang: "Mammalian target of rapamycin up-regulation of pyruvate kinase isoenzyme type M2 is critical for aerobic glycolysis and tumor growth." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 10, pp. 4129-34, (2011) (PubMed).

Target Details

Target: PKM1

Background: PKM1 is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. The protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.

Molecular Weight: 57937

Gene ID: 5315

NCBI Accession: NP_001193725, NP_001193726, NP_001193727, NP_001193728, NP_002645, NP_872270, NP_872271

UniProt: P14618