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PNPLA3 antibody (Internal Region)

Verified PNPLA3 Reactivity: Human, Mouse WB, ELISA, IHC Host: Goat Polyclonal unconjugated
Catalog No. ABIN361184
  • Target See all PNPLA3 Antibodies
    PNPLA3 (Patatin-Like phospholipase Domain Containing 3 (PNPLA3))
    Binding Specificity
    • 15
    • 11
    • 9
    • 9
    • 8
    • 7
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 34
    • 32
    • 3
    • 1
    Human, Mouse
    Host
    • 58
    • 3
    • 2
    Goat
    Clonality
    • 63
    Polyclonal
    Conjugate
    • 26
    • 7
    • 6
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PNPLA3 antibody is un-conjugated
    Application
    • 58
    • 37
    • 13
    • 13
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Purpose
    Pnpla3 / Adiponutrin (mouse)
    Sequence
    EHDICPKVKS TN
    Specificity
    This antibody is raised against a peptide derived from the Mouse protein sequence and this peptide differs from Human only in position 2.
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence EHDICPKVKSTN, from the internal region of the protein sequence according to NP_473429.2.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 8 μg/mL.

    Western Blot: Approx 45 kDa band observed in Mouse Adrenal Gland lysates, and also in preliminary testing of Mouse and Human Kidney and Rat Adrenal Gland lysate (calculated MW of 42.6Da according to Mouse NP_473429.2). Recommended concentration: 0.01-0.03

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Yang, Mottillo, Mladenovic-Lucas, Zhou, Granneman: "Dynamic interactions of ABHD5 with PNPLA3 regulate triacylglycerol metabolism in brown adipocytes." in: Nature metabolism, Vol. 1, Issue 5, pp. 560-569, (2019) (PubMed).

    Hao, Ito, Huang, Sae-tan, Lambert, Ross: "Shifts in dietary carbohydrate-lipid exposure regulate expression of the non-alcoholic fatty liver disease-associated gene PNPLA3/adiponutrin in mouse liver and HepG2 human liver cells." in: Metabolism: clinical and experimental, Vol. 63, Issue 10, pp. 1352-62, (2014) (PubMed).

    Kumari, Schoiswohl, Chitraju, Paar, Cornaciu, Rangrez, Wongsiriroj, Nagy, Ivanova, Scott, Knittelfelder, Rechberger, Birner-Gruenberger, Eder, Brown, Haemmerle, Oberer, Lass, Kershaw, Zimmermann et al.: "Adiponutrin functions as a nutritionally regulated lysophosphatidic acid acyltransferase. ..." in: Cell metabolism, Vol. 15, Issue 5, pp. 691-702, (2012) (PubMed).

    Gustavsson, Soga, Wahlström, Vesterlund, Azimi, Norstedt, Tollet-Egnell: "Sex-dependent hepatic transcripts and metabolites in the development of glucose intolerance and insulin resistance in Zucker diabetic fatty rats." in: Journal of molecular endocrinology, Vol. 47, Issue 2, pp. 129-43, (2011) (PubMed).

  • Target
    PNPLA3 (Patatin-Like phospholipase Domain Containing 3 (PNPLA3))
    Alternative Name
    Pnpla3 (PNPLA3 Products)
    Synonyms
    ADPN antibody, C22orf20 antibody, iPLA(2)epsilon antibody, Adpn antibody, patatin like phospholipase domain containing 3 antibody, patatin-like phospholipase domain containing 3 antibody, PNPLA3 antibody, Pnpla3 antibody
    Background
    Pnpla3, patatin-like phospholipase domain containing 3, Adpn, adiponutrin
    Molecular Weight
    42.6kDa
    Gene ID
    80339, 116939, 362972
    NCBI Accession
    NP_473429
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