mEOS2 antibody

Catalog No. ABIN1110898

Product Details

Target: mEOS2

Reactivity: Lobophyllia hemprichii

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA

Specificity: This antibody binds specifically to mEos2 GFP.

Purification: Affinity Purified

Purity: The IgG fraction of tissue culture supernatant was purified by Protein A / G affinity chromatography.

Immunogen: The immunogen was recombinant mEos2 GFP. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen.

Clone: 12C11-C8

Isotype: IgG2a

Application Details

Application Notes: For ELISA we recommend a work dilution of 1: 64,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the antibody with distilled or de-ionized water to a final concentration of 1 mg/ml.

Buffer: Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives.

Storage Comment: Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can also be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 30 days at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.

References

Zhang, Chang, Zhang, Yu, Wu, Ji, Chen, Liu, Lu, Liu, Zhang, Xu, Xu: "Rational design of true monomeric and bright photoactivatable fluorescent proteins." in: Nature methods, Vol. 9, Issue 7, pp. 727-9, (2012) (PubMed).

Target Details

Target: mEOS2

Background: Monomeric (m)Eos2 is an engineered photoactivatable fluorescent protein widely used for superresolution microscopy. An optical highlighter isolated from another stony coral (Lobophyllia hemprichii), the tetrameric EosFP (named after the goddess of dawn in Greek mythology), behaves similarly to Kaede and the other variants described above. EosFP emits bright green fluorescence at 516 nanometers, and can be photoconverted to orange-red (581 nanometers) fluorescence when illuminated at near-ultraviolet wavelengths. Random and site-directed mutagenesis of tetrameric EosFP was used to generate two dimers and a true monomeric protein named mEosFP.