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Nanog antibody (Internal Region)

Verified NANOG Reactivity: Human WB, ELISA, IF, IHC Host: Goat Polyclonal unconjugated
Catalog No. ABIN185444
  • Target See all Nanog (NANOG) Antibodies
    Nanog (NANOG) (Nanog Homeobox (NANOG))
    Binding Specificity
    • 16
    • 16
    • 12
    • 10
    • 8
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 137
    • 59
    • 23
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 128
    • 15
    • 2
    • 1
    Goat
    Clonality
    • 122
    • 24
    Polyclonal
    Conjugate
    • 76
    • 10
    • 9
    • 9
    • 8
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    This Nanog antibody is un-conjugated
    Application
    • 115
    • 64
    • 48
    • 46
    • 26
    • 26
    • 23
    • 18
    • 18
    • 17
    • 8
    • 7
    • 5
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)
    Purpose
    NANOG
    Sequence
    QNQRMKSKRW QKNN
    Cross-Reactivity
    Dog, Human, Pig
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-QNQRMKSKRWQKNN, from the internal region of the protein sequence according to NP_079141.2.
    Isotype
    IgG
    Top Product
    Discover our top product NANOG Primary Antibody
  • Application Notes

    Immunohistochemistry: This antibody was successfully used in: Vassena et al, Hum Mol Genet. 2012 Aug 1,21(15):3366-73. PMID: 22547223, Sancho-Martinez et al, Nat Commun. 2016 Feb 22,7:10743. PMID: 26899176, and Montserrat et al, Cell Transplant. 2012,21(

    Western Blot: Approx 38 kDa band observed in Human Ovary lysates (calculated MW of 34.6 kDa according to NP_079141.2). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Comment

    IF/ICC: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green).
    This antibody was successfully used in IF on Human: PMID: 30878013, 21681858, 22613719, 21877920. 21285354,19890879, 19483

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Sancho-Martinez, Nivet, Xia, Hishida, Aguirre, Ocampo, Ma, Morey, Krause, Zembrzycki, Ansorge, Vazquez-Ferrer, Dubova, Reddy, Lam, Hishida, Wu, Esteban, OLeary, Wahl, Verma, Laurent, Izpisua Belmonte: "Establishment of human iPSC-based models for the study and targeting of glioma initiating cells." in: Nature communications, Vol. 7, pp. 10743, (2016) (PubMed).

    Tolar, McGrath, Xia, Riddle, Lees, Eide, Keene, Liu, Osborn, Lund, Blazar, Wagner: "Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa." in: The Journal of investigative dermatology, Vol. 134, Issue 5, pp. 1246-54, (2014) (PubMed).

    Vassena, Montserrat, Carrasco Canal, Aran, de Oñate, Veiga, Izpisua Belmonte: "Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells." in: Human molecular genetics, Vol. 21, Issue 15, pp. 3366-73, (2012) (PubMed).

    Montserrat, Ramírez-Bajo, Xia, Sancho-Martinez, Moya-Rull, Miquel-Serra, Yang, Nivet, Cortina, González, Izpisua Belmonte, Campistol: "Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2." in: The Journal of biological chemistry, Vol. 287, Issue 29, pp. 24131-8, (2012) (PubMed).

    Montserrat, de Oñate, Garreta, González, Adamo, Eguizábal, Häfner, Vassena, Izpisua Belmonte: "Generation of feeder-free pig induced pluripotent stem cells without Pou5f1." in: Cell transplantation, Vol. 21, Issue 5, pp. 815-25, (2012) (PubMed).

    Montserrat, Garreta, González, Gutiérrez, Eguizábal, Ramos, Borrós, Izpisua Belmonte: "Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system." in: The Journal of biological chemistry, Vol. 286, Issue 14, pp. 12417-28, (2011) (PubMed).

    Montserrat, Bahima, Batlle, Häfner, Rodrigues, González, Izpisúa Belmonte: "Generation of pig iPS cells: a model for cell therapy." in: Journal of cardiovascular translational research, Vol. 4, Issue 2, pp. 121-30, (2011) (PubMed).

    Eguizabal, Montserrat, Vassena, Barragan, Garreta, Garcia-Quevedo, Vidal, Giorgetti, Veiga, Izpisua Belmonte: "Complete meiosis from human induced pluripotent stem cells." in: Stem cells (Dayton, Ohio), Vol. 29, Issue 8, pp. 1186-95, (2011) (PubMed).

    Rodríguez-Pizà, Richaud-Patin, Vassena, González, Barrero, Veiga, Raya, Izpisúa Belmonte: "Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 1, pp. 36-44, (2010) (PubMed).

    Raya, Rodríguez-Pizà, Guenechea, Vassena, Navarro, Barrero, Consiglio, Castellà, Río, Sleep, González, Tiscornia, Garreta, Aasen, Veiga, Verma, Surrallés, Bueren, Izpisúa Belmonte: "Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells." in: Nature, Vol. 460, Issue 7251, pp. 53-9, (2009) (PubMed).

  • Target
    Nanog (NANOG) (Nanog Homeobox (NANOG))
    Alternative Name
    NANOG (NANOG Products)
    Synonyms
    2410002E02Rik antibody, ENK antibody, ecat4 antibody, NANOG antibody, hacp antibody, wu:fd19e04 antibody, wu:fd20a07 antibody, zgc:193933 antibody, Nanog homeobox antibody, nanog homeobox antibody, Nanog antibody, NANOG antibody, nanog antibody
    Background
    NANOG, Nanog homeobox, HGNC:20857 , homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
    Gene ID
    79923, 71950, 414065
    NCBI Accession
    NP_079141
    Pathways
    Stem Cell Maintenance
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