DYKDDDDK Tag Peptide

Catalog No. ABIN399388

Product Details

Target: DYKDDDDK Tag

Purity: > 95 %

Application Details

Restrictions: For Research Use only

Handling

Storage: 4 °C

References

Alonso-de Vega, Martín, Smits: "USP7 controls Chk1 protein stability by direct deubiquitination." in: Cell cycle (Georgetown, Tex.), Vol. 13, Issue 24, pp. 3921-6, (2015) (PubMed).

Sathiyamoorthy, Jiang, Hu, Rowe, Möhl, Chen, Jiang, Mellins, Longnecker, Zhou, Jardetzky: "Assembly and architecture of the EBV B cell entry triggering complex." in: PLoS pathogens, Vol. 10, Issue 8, pp. e1004309, (2014) (PubMed).

Inoue, Li, Schnell: "An essential role for chloroplast heat shock protein 90 (Hsp90C) in protein import into chloroplasts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 8, pp. 3173-8, (2013) (PubMed).

Verghese, Morano: "A lysine-rich region within fungal BAG domain-containing proteins mediates a novel association with ribosomes." in: Eukaryotic cell, Vol. 11, Issue 8, pp. 1003-11, (2012) (PubMed).

Das, Khosla: "In vivo and in vitro analysis of the hedamycin polyketide synthase." in: Chemistry & biology, Vol. 16, Issue 11, pp. 1197-207, (2009) (PubMed).

Target Details

Target: DYKDDDDK Tag

Target Type: Tag

Background: A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free DYKDDDDK peptide.