Abeta-pE3 antibody

Catalog No. ABIN1742435

Product Details

Target: Abeta-pE3

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for Abeta-pE3.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide pyr-EFRHD coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500
IHC-P: 1 : 200

Comment:

WB: Detects purified Abeta pE3. Complex samples like brain extracts still have to be tested.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References

Morawski, Schilling, Kreuzberger, Waniek, Jäger, Koch, Cynis, Kehlen, Arendt, Hartlage-Rübsamen, Demuth, Roßner: "Glutaminyl cyclase in human cortex: correlation with (pGlu)-amyloid-? load and cognitive decline in Alzheimer's disease." in: Journal of Alzheimer's disease : JAD, Vol. 39, Issue 2, pp. 385-400, (2014) (PubMed).

Becker, Kohlmann, Alexandru, Jagla, Canneva, Bäuscher, Cynis, Sedlmeier, Graubner, Schilling, Demuth, von Hörsten: "Glutaminyl cyclase-mediated toxicity of pyroglutamate-beta amyloid induces striatal neurodegeneration." in: BMC neuroscience, Vol. 14, pp. 108, (2013) (PubMed).

Nussbaum, Schilling, Cynis, Silva, Swanson, Wangsanut, Tayler, Wiltgen, Hatami, Rönicke, Reymann, Hutter-Paier, Alexandru, Jagla, Graubner, Glabe, Demuth, Bloom: "Prion-like behaviour and tau-dependent cytotoxicity of pyroglutamylated amyloid-β." in: Nature, Vol. 485, Issue 7400, pp. 651-5, (2012) (PubMed).

Morawski, Brückner, Jäger, Seeger, Matthews, Arendt: "Involvement of perineuronal and perisynaptic extracellular matrix in Alzheimer's disease neuropathology." in: Brain pathology (Zurich, Switzerland), Vol. 22, Issue 4, pp. 547-61, (2012) (PubMed).

Hartlage-Rübsamen, Morawski, Waniek, Jäger, Zeitschel, Koch, Cynis, Schilling, Schliebs, Demuth, Rossner: "Glutaminyl cyclase contributes to the formation of focal and diffuse pyroglutamate (pGlu)-A? deposits in hippocampus via distinct cellular mechanisms." in: Acta neuropathologica, Vol. 121, Issue 6, pp. 705-19, (2011) (PubMed).

Alexandru, Jagla, Graubner, Becker, Bäuscher, Kohlmann, Sedlmeier, Raber, Cynis, Rönicke, Reymann, Petrasch-Parwez, Hartlage-Rübsamen, Waniek, Rossner, Schilling, Osmand, Demuth, von Hörsten: "Selective hippocampal neurodegeneration in transgenic mice expressing small amounts of truncated Aβ is induced by pyroglutamate-Aβ formation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 36, pp. 12790-801, (2011) (PubMed).

Target Details

Target: Abeta-pE3

Background: Synonyms: pyro-Glu Abeta, pE-Abeta, pGlu-Abeta, Abeta(3(pE)-40/42)