ToxinEraser Endotoxin Removal Resin

Catalog No. ABIN491525

Product Details

Application: Affinity Chromatography (AC)

Brand: ToxinEraser™

Characteristics: ToxinEraser is an endotoxin removal resin of high efficiency. It is based on the affinity matrix of modified polymyxin B (PMB), which allows highly efficient endotoxin removal. The final endotoxin level can be decreased to less than 0.1 EU/mL with repeat use of ToxinEraser endotoxin removal resin.

• High stability and high removal efficiency
• High binding capacity: > 2,000,000 EU/mL (CV)
• Fast flow without any constant speed pump
• Reusable up to five times if properly regenerated

• Size: 1.5 mL in pre-packed column
• Binding Capacity: Up to 2,000,000 EU/mL resin.
• Ligand: Modified PMB (Polymixin B)
• pH Stability: pH 5 -10
• Support Matrix: 4% cross-linked agarose, spherical beads
• Mean Particle Size: 90 μm
• Equilibration Buffer: Phosphate-Buffer, pH 8.0
• Types of substances that can be applied to the column: Protein, including peptides and antibody, polysaccharide etc.
• Applicable Ionic Strength: 0.1 to 0.5 M NaCl
• Substances tested that do not interfere with performance: 20% DMSO, 20% ethanol, 20% glycerol, 1 M urea, 300 mM imidazole, 0.05% Tween 20, 10 mM DTT, etc.

Material not included:
- Column and column stand
- 0.1 N sodium hydroxide or 0.1 N hydrochloric acid for adjusting the pH of the sample.
- Regeneration Buffer. For regeneration of the resin, Regeneration Buffer (ABIN769949) is needed.
- Equilibration Buffer, customer can use any proper buffer to equilibrate the resin. A pH of 7-8 and the salt concentration of 0.15-0.5 M NaCl are recommended for best results.
- Equilibration Buffer is also available (ABIN769950).

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 50 % slurry

Handling Advice: Do not freeze

Storage: 4 °C

References

Hao, Zhang, Liang, Song, Hou: "rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner." in: Cellular & molecular immunology, Vol. 11, Issue 2, pp. 206-14, (2014) (PubMed).

Liu, Ferrer, Konomos, Ford: "Inhibition of CD8+ T cell-derived CD40 signals is necessary but not sufficient for Foxp3+ induced regulatory T cell generation in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 4, pp. 1957-64, (2013) (PubMed).

Jheng, Tsai, Chen, Lin, Chyan, Yang, Lin: "Recombinant dioscorins of the yam storage protein expressed in Escherichia coli exhibit antioxidant and immunomodulatory activities." in: Protein expression and purification, Vol. 85, Issue 1, pp. 77-85, (2012) (PubMed).

Liu, Xu, Lin, Tian, Ji, Xia, Xu, Yin, Zhang, Jiao, Wang, Xu, Shao: "PTD-hFOXP3 protein acts as an immune regulator to convert human CD4(+)CD25(-) T cells to regulatory T-like cells." in: Journal of cellular biochemistry, Vol. 113, Issue 12, pp. 3797-809, (2012) (PubMed).

Zhu, Liu, Lu, Long, Shi, Yu: "Construction, purification, and evaluation of multivalent DNA vaccine against Schistosoma japonicum." in: Parasitology research, Vol. 108, Issue 1, pp. 115-21, (2011) (PubMed).

Target Details

Background: Lipopolysaccharide (LPS) is a bacterial endotoxin and a major constituent of the cell walls of gram-negative bacteria. During gram-negative bacteremia or endotoxemia, LPS is the principal pathophysiological mediator by which bacteria can cause hypotension, organ failure, disseminated intravascular coagulation, or fatal shock in mammalian hosts. The removal of these endotoxins is highly necessary for downstream processes.

The ToxinEraserTM Endotoxin Removal Resin utilizes immobilized polymixin B to bind and remove pyrogens from solution. The polymixins are a family of antibiotics that contain a cationic cyclopeptide with a fatty acid chain. Polymixin B neutralizes the biological activity of endotoxins by binding to the lipid A portion of bacterial lipopolysaccharide.