S100A9 Protein (AA 1-114) (GST tag)

Catalog No. ABIN1319076

Product Details

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Protein Type: Recombinant

Protein Characteristics: AA 1-114

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This S100A9 protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Purpose: S100A9 (Human) Recombinant Protein (P01)

Sequence: MTCKMSQLER NIETIINTFH QYSVKLGHPD TLNQGEFKEL VRKDLQNFLK KENRNEKVIE HIMEDLDTNA DKQLSFEEFI MLMARLTWAS HEKMHEGDEG PGHHHKPGLG EGTP

Characteristics: Human S100A9 full-length ORF ( AAH47681, 1 a.a. - 114 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Shirley, von Maltzan, Robbins, Kusewitt: "Melanocyte and melanoma cell activation by calprotectin." in: Journal of skin cancer, Vol. 2014, pp. 846249, (2014) (PubMed).

Maiseyeu, Badgeley, Kampfrath, Mihai, Deiuliis, Liu, Sun, Parthasarathy, Simon, Croce, Rajagopalan: "In vivo targeting of inflammation-associated myeloid-related protein 8/14 via gadolinium immunonanoparticles." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 32, Issue 4, pp. 962-70, (2012) (PubMed).

Feng, Lee, Chang, Chan, Kuo, Lin, Chung, Kuo, Yu, Lin, Wang, Chou, Huang, Kuo: "CD14(+)S100A9(+) monocytic myeloid-derived suppressor cells and their clinical relevance in non-small cell lung cancer." in: American journal of respiratory and critical care medicine, Vol. 186, Issue 10, pp. 1025-36, (2012) (PubMed).

Saha, Lee, Zhang, Chandra, Su, Mukherjee: "Lack of an endogenous anti-inflammatory protein in mice enhances colonization of B16F10 melanoma cells in the lungs." in: The Journal of biological chemistry, Vol. 285, Issue 14, pp. 10822-31, (2010) (PubMed).

Venkataraman, Cole, Svoboda, Pohl, Cole: "Cationic polypeptides are required for anti-HIV-1 activity of human vaginal fluid." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 11, pp. 7560-7, (2005) (PubMed).

Target Details

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Alternative Name: S100A9 (S100A9 Products)

Background: Full Gene Name: S100 calcium binding protein A9
Synonyms: 60B8AG,CAGB,CFAG,CGLB,L1AG,LIAG,MAC387,MIF,MRP14,NIF,P14

Gene ID: 6280

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins