Cyclin A Protein (AA 1-432) (GST tag)
(1 reference)-
- Target See all Cyclin A (CCNA2) Proteins
- Cyclin A (CCNA2) (Cyclin A2 (CCNA2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-432
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This Cyclin A protein is labelled with GST tag.
- Application
- ELISA, Western Blotting (WB), Antibody Array (AA), Affinity Purification (AP)
- Purpose
- CCNA2 (Human) Recombinant Protein (P01)
- Sequence
- MLGNSAPGPA TREAGSALLA LQQTALQEDQ ENINPEKAAP VQQPRTRAAL AVLKSGNPRG LAQQQRPKTR RVAPLKDLPV NDEHVTVPPW KANSKQPAFT IHVDEAEKEA QKKPAESQKI EREDALAFNS AISLPGPRKP LVPLDYPMDG SFESPHTMDM SIVLEDEKPV SVNEVPDYHE DIHTYLREME VKCKPKVGYM KKQPDITNSM RAILVDWLVE VGEEYKLQNE TLHLAVNYID RFLSSMSVLR GKLQLVGTAA MLLASKFEEI YPPEVAEFVY ITDDTYTKKQ VLRMEHLVLK VLTFDLAAPT VNQFLTQYFL HQQPANCKVE SLAMFLGELS LIDADPYLKY LPSVIAGAAF HLALYTVTGQ SWPESLIRKT GYTLESLKPC LMDLHQTYLK APQHAQQSIR EKYKNSKYHG VSLLNPPETL NL
- Characteristics
- Human CCNA2 full-length ORF ( NP_001228.1, 1 a.a. - 432 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Cyclin A2 (CCNA2) (AA 268-432) protein (His-IF2DI Tag)
CCNA2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Cyclin A2 (CCNA2) (AA 144-264) protein (His-IF2DI Tag)CCNA2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Cyclin A2 (CCNA2) (AA 1-100) protein (GST tag)CCNA2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Cyclin A2 (CCNA2) (AA 1-422) protein (His tag)Crystallography grade CCNA2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Cyclin A2 (CCNA2) protein (Myc-DYKDDDDK Tag)CCNA2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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-
: "Cyclin A2 is required for sister chromatid segregation, but not separase control, in mouse oocyte meiosis." in: Cell reports, Vol. 2, Issue 5, pp. 1077-87, (2012) (PubMed).
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: "Cyclin A2 is required for sister chromatid segregation, but not separase control, in mouse oocyte meiosis." in: Cell reports, Vol. 2, Issue 5, pp. 1077-87, (2012) (PubMed).
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- Target
- Cyclin A (CCNA2) (Cyclin A2 (CCNA2))
- Alternative Name
- CCNA2 (CCNA2 Products)
- Synonyms
- CCN1 Protein, CCNA Protein, AA408589 Protein, Ccn-1 Protein, Ccn1 Protein, Ccna Protein, CycA2 Protein, Cyca Protein, cb671 Protein, chunp6924 Protein, wu:fi36f03 Protein, CYCA Protein, Cyclin-A2 Protein, ccna2 Protein, ccn1 Protein, ccna Protein, ccna1 Protein, Cyclin-A Protein, cyclinA Protein, cyclin A2 Protein, cyclin A2 S homeolog Protein, cyclin A2 L homeolog Protein, CCNA2 Protein, Ccna2 Protein, ccna2 Protein, ccna2.S Protein, ccna2.L Protein
- Background
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Full Gene Name: cyclin A2
Synonyms: CCN1,CCNA - Gene ID
- 890
- NCBI Accession
- NM_001237
- Pathways
- PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
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