Fluorescent Dopamine Neurotransmitter Ligand

Catalog No. ABIN1059102

Product Details

Specificity: The fluorescent labeled catecholamine neurotransmitter analog of dopamine (Product Name: DnsylD-1TM), with dansyl attached by a 5-carbon spacer arm to dopamine, provides a unique pharmacological tool. This dansyl conjugate of dopamine offers a stable, receptor selective and high affinity pharmacological ligand that can be detected by fluorescent microscopy and FLIPR devices. Unlike recently reported synthesized fluorescent neurotransmitter analogs, DnsylD-1TM contains the entire dopamine structural moiety attached with the 5-carbon polylinker to the small fluorescence label dansyThis structural modification minimizes steric hindrance and maintains high receptor affinity.
A large stokes shift between excitation and emission maxima (Ex:Em, 313:515 nm) mediated by the dansyl moiety attached to a carbon poly-linker to dopamine offers a high signal to noise detection with minimal autofluorescence. The dansyl moiety changes spectral characteristics on intermolecular contact, facilitating biophysical characterization of ligand-receptor interaction. The DnsylD-1 reagent also provides a suitable substitute for radioactive ligand.

Purity: > 98 %

Application Details

Application Notes: Applications for Fluorescent Dopamine Ligand-Neurotransmitter (DnsylD-1TM):
DnsylD-1TM has the below applicability due to: the small molecular size of the dansyl fluorescent group, a long spacer arm between ligand and the fluorescent tag, stability in aqueous solution, low autofluorescence of the surrounding cellular or tissue assay medium due to the long separation between excitation and emission maxima of danysl attached to the 5-C spacer arm to dopamine.
Histochemical imaging and quantitation of dopamine receptor expression in vivo and vitro.
Pharmacological applications as a substitute for radioactive ligand.
Dopamine metabolism and turnover analysis.
Dopamine uptake analysis.
Analysis of disease conditions associated with altered dopamine synthesis, dopamine receptor expression or dopamine uptake: human disease research applicability in the following: a) Parkinson's disease. b) Psychological disorder, including ADHD, schizophrenia and substance abuse. c) Physiological regulation of cardiovascular, renal and immune function.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Solubility: DMSO

Concentration: 2 µM

Storage: 4 °C

References

May, Andreasson-Ochsner, Fu, Low, Tan, de Hoog, Ritz, Nallani, Sinner: "In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies." in: Angewandte Chemie (International ed. in English), Vol. 52, Issue 2, pp. 749-53, (2013) (PubMed).

Target Details

Molecular Weight: 499.62 Da