PIP4K2A antibody (C-Term)

Catalog No. ABIN392598

Product Details anti-PIP4K2A Antibody

Target: PIP4K2A (Phosphatidylinositol-5-Phosphate 4-Kinase, Type II, alpha (PIP4K2A))

Binding Specificity: AA 303-335, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIP4K2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: M, Pig, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PIP4K2 Alpha (PIP4K2A) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-335 amino acids from the C-terminal region of human PIP4K2 Alpha (PIP4K2A).

Clone: RB1741

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PIP4K2A antibody (ABIN392598)

Jones, Foulger, Keune, Bultsma, Divecha: "PtdIns5P is an oxidative stress-induced second messenger that regulates PKB activation." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 27, Issue 4, pp. 1644-56, (2013) (PubMed).

Keune, Jones, Bultsma, Sommer, Zhou, Lu, Divecha: "Regulation of phosphatidylinositol-5-phosphate signaling by Pin1 determines sensitivity to oxidative stress." in: Science signaling, Vol. 5, Issue 252, pp. ra86, (2012) (PubMed).

Huang, Anderson, Cao, Wiechmann, Rajala: "Light-induced tyrosine phosphorylation of rod outer segment membrane proteins regulate the translocation, membrane binding and activation of type II ? phosphatidylinositol-5-phosphate 4-kinase." in: Neurochemical research, Vol. 36, Issue 4, pp. 627-35, (2011) (PubMed).

Bultsma, Keune, Divecha: "PIP4Kbeta interacts with and modulates nuclear localization of the high-activity PtdIns5P-4-kinase isoform PIP4Kalpha." in: The Biochemical journal, Vol. 430, Issue 2, pp. 223-35, (2010) (PubMed).

Wilcox, Hinchliffe: "Regulation of extranuclear PtdIns5P production by phosphatidylinositol phosphate 4-kinase 2alpha." in: FEBS letters, Vol. 582, Issue 9, pp. 1391-4, (2008) (PubMed).

Target Details for PIP4K2A

Target: PIP4K2A (Phosphatidylinositol-5-Phosphate 4-Kinase, Type II, alpha (PIP4K2A))

Alternative Name: PIP5K2 Alpha (PIP5K2A) (PIP4K2A Products)

Synonyms: PI5P4KA antibody, PIP5K2A antibody, PIP5KII-alpha antibody, PIP5KIIA antibody, PIPK antibody, AW742916 antibody, Pip5k2a antibody, pipk antibody, pi5p4ka antibody, pip5k2a antibody, pip5kiia antibody, pip4k2a antibody, zgc:194746 antibody, zgc:194777 antibody, phosphatidylinositol-5-phosphate 4-kinase type 2 alpha antibody, phosphatidylinositol-5-phosphate 4-kinase, type II, alpha antibody, phosphatidylinositol-5-phosphate 4-kinase, type II, alpha S homeolog antibody, phosphatidylinositol-5-phosphate 4-kinase, type II, alpha a antibody, PIP4K2A antibody, Pip4k2a antibody, pip4k2a.S antibody, pip4k2a antibody, pip4k2aa antibody

Background: Phosphatidylinositol-4,5-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. TPIP5K2A is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-4-phosphate on the fifth hydroxyl of the myo-inositol ring to form phosphatidylinositol-4,5-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. The protein is a member of the phosphatidylinositol-4-phosphate 5-kinase family.

Molecular Weight: 46225

Gene ID: 5305

NCBI Accession: NP_005019

UniProt: P48426

Pathways: Inositol Metabolic Process