ASCL2 antibody (Middle Region)

Catalog No. ABIN2779425

Product Details anti-ASCL2 Antibody

Target: ASCL2 (Achaete-Scute Complex Homolog 2 (ASCL2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASCL2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: QALRQHVPHG GASKKLSKVE TLRSAVEYIR ALQRLLAEHD AVRNALAGGL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 92%, Rabbit: 77%, Rat: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against ASCL2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ASCL2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 193 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ASCL2 antibody (ABIN2779425)

Schwitalla, Fingerle, Cammareri, Nebelsiek, Göktuna, Ziegler, Canli, Heijmans, Huels, Moreaux, Rupec, Gerhard, Schmid, Barker, Clevers, Lang, Neumann, Kirchner, Taketo, van den Brink, Sansom, Arkan et al.: "Intestinal tumorigenesis initiated by dedifferentiation and acquisition of stem-cell-like properties. ..." in: Cell, Vol. 152, Issue 1-2, pp. 25-38, (2013) (PubMed).

Target Details for ASCL2

Target: ASCL2 (Achaete-Scute Complex Homolog 2 (ASCL2))

Alternative Name: ASCL2 (ASCL2 Products)

Synonyms: ASH2 antibody, HASH2 antibody, MASH2 antibody, bHLHa45 antibody, ASCL2 antibody, 2410083I15Rik antibody, Mash2 antibody, achaete-scute family bHLH transcription factor 2 antibody, ASCL2 antibody, Ascl2 antibody

Background: AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.This gene is a member of the basic helix-loop-helix (BHLH) family of transcription factors. It activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. Involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1649 BC028140.1 1-1649 1650-1864 BC057801.1 1286-1500
Alias Symbols: ASH2, HASH2, MASH2, bHLHa45
Protein Interaction Partner: EP300, APEX1, CALM3, CALM1, HCFC1, TUBB, KMT2C, NCOA6, TUBA4A, RBBP5,
Protein Size: 193

Molecular Weight: 20 kDa

Gene ID: 430

NCBI Accession: NM_005170, NP_005161

UniProt: Q99929

Pathways: Stem Cell Maintenance