psi-D Subunit of Photosystem I antibody

Catalog No. ABIN5684022

Product Details

Target: psi-D Subunit of Photosystem I

Reactivity: Chlamydomonas, Oryza sativa, Spinach, Zea mays

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Cross-Reactivity (Details): No cross-reactivity with: Synechococcus elongatus

Characteristics: Expected / apparent Molecular Weight of the Antigene: 17.9 / 20 (for Arabidopsis thaliana)
PsaD has frequently been used as a marker for intact PSI reaction centers.

Purification: serum

Immunogen: KLH-conjugated synthetic peptide 100% conserved in all known plant PsaD sequences including Arabidopsis thaliana (At1g03130 and At1g02770) as well as Physcomitrella patens. The conservation in Chlamydomonas reinhardtii is high (14 of 16 aminoacids are identical).

Application Details

Application Notes: Recommended Dilution: 1 : 1000 with SuperSignal West Pico detection (WB).

Comment:

this antibody is a replacement for former product, anti-PsaD AS04 046

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References

Heinnickel, Alric, Wittkopp, Yang, Catalanotti, Dent, Niyogi, Wollman, Grossman: "Novel thylakoid membrane GreenCut protein CPLD38 impacts accumulation of the cytochrome b6f complex and associated regulatory processes." in: The Journal of biological chemistry, Vol. 288, Issue 10, pp. 7024-36, (2013) (PubMed).

Yadavalli, Neelam, Rao, Reddy, Subramanyam: "Differential degradation of photosystem I subunits under iron deficiency in rice." in: Journal of plant physiology, Vol. 169, Issue 8, pp. 753-9, (2012) (PubMed).

Lang, Mueller, Hoernstein, Porankiewicz-Asplund, Vervliet-Scheebaum, Reski: "Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics." in: Plant cell reports, Vol. 30, Issue 2, pp. 205-15, (2011) (PubMed).

Target Details

Target: psi-D Subunit of Photosystem I

Alternative Name: PSI-D subunit of photosystem I (PsaD)

Background: AGI Code: At1g03130
PsaD (PSI-D) is a core subunit of photosystem I highly conserved in all photosynthetic organisms (including bacteria with Fe-S type reaction centers). In eukaryots its encoded by 1 to 2 nuclear gene(s) and imported as a precursor into the chloroplast. In the thylakoid membrane it associates with PsaA and PsaB on the stromal site of the PSI core forming the Fd-docking site. PsaD is also required for the stable assembly of PsaC.

Molecular Weight: expected: 17.9 kDa, apparent: 20 (for Arabidopsis thaliana)

UniProt: Q9S7H1, Q9SA56