ATP5B Protein (AA 1-529) (GST tag)

Catalog No. ABIN1346052

Product Details

Target: ATP5B (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B))

Protein Type: Recombinant

Protein Characteristics: AA 1-529

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This ATP5B protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Purpose: ATP5B (Human) Recombinant Protein (P01)

Sequence: MLGFVGRVAA APASGALRRL TPSASLPPAQ LLLRAAPTAV HPVRDYAAQT SPSPKAGAAT GRIVAVIGAV VDVQFDEGLP PILNALEVQG RETRLVLEVA QHLGESTVRT IAMDGTEGLV RGQKVLDSGA PIKIPVGPET LGRIMNVIGE PIDERGPIKT KQFAPIHAEA PEFMEMSVEQ EILVTGIKVV DLLAPYAKGG KIGLFGGAGV GKTVLIMELI NNVAKAHGGY SVFAGVGERT REGNDLYHEM IESGVINLKD ATSKVALVYG QMNEPPGARA RVALTGLTVA EYFRDQEGQD VLLFIDNIFR FTQAGSEVSA LLGRIPSAVG YQPTLATDMG TMQERITTTK KGSITSVQAI YVPADDLTDP APATTFAHLD ATTVLSRAIA ELGIYPAVDP LDSTSRIMDP NIVGSEHYDV ARGVQKILQD YKSLQDIIAI LGMDELSEED KLTVSRARKI QRFLSQPFQV AEVFTGHMGK LVPLKETIKG FQQILAGEYD HLPEQAFYMV GPIEEAVAKA DKLAEEHSS

Characteristics: Human ATP5B full-length ORF ( NP_001677.2, 1 a.a. - 529 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Hansson, Korsgren, Pontén, Korsgren: "Enteroviruses and the pathogenesis of type 1 diabetes revisited: cross-reactivity of enterovirus capsid protein (VP1) antibodies with human mitochondrial proteins." in: The Journal of pathology, Vol. 229, Issue 5, pp. 719-28, (2013) (PubMed).

Rubel, Schisler, Hamlett, DeKroon, Gautel, Alzate, Patterson: "Diggin' on u(biquitin): a novel method for the identification of physiological E3 ubiquitin ligase substrates." in: Cell biochemistry and biophysics, Vol. 67, Issue 1, pp. 127-38, (2013) (PubMed).

Target Details

Target: ATP5B (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B))

Alternative Name: ATP5B (ATP5B Products)

Synonyms: atpmb Protein, atpsb Protein, Atpsyn-beta Protein, hm:zehn0534 Protein, im:6793121 Protein, wu:fj38d01 Protein, zgc:111961 Protein, ATPMB Protein, ATPSB Protein, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide Protein, ATP synthase subunit beta, mitochondrial Protein, ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit Protein, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide S homeolog Protein, ATP5B Protein, atp5b Protein, Atp5b Protein, LOC100401662 Protein, LOC100635763 Protein, atp5b.S Protein

Background: Full Gene Name: ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Synonyms: ATPMB,ATPSB,MGC5231

Gene ID: 506

NCBI Accession: NM_001686

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process