V5 Epitope Tag antibody (HRP)

Catalog No. ABIN1573948

Product Details anti-V5 Epitope Tag Antibody (HRP)

Target: V5 Epitope Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This V5 Epitope Tag antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Sequence: GKPIPNPLLG LDST

Specificity: Rabbit Anti-V5-tag [HRP] Polyclonal Antibody specifically recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.

Purification: Immunoaffinity chromatography

Immunogen: V5 epitope tag peptide GKPIPNPLLGLDST conjugated - KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-V5 Epitope Tag antibody (HRP) (ABIN1573948)

Dahl, Corydon, Ränkel, Nielsen, Füchtbauer, Knudsen: "An eEF1A1 truncation encoded by PTI-1 exerts its oncogenic effect inside the nucleus." in: Cancer cell international, Vol. 14, Issue 1, pp. 17, (2014) (PubMed).

Tahir, Yang, Goltsov, Song, Ren, Wang, Chang, Thompson: "Caveolin-1-LRP6 signaling module stimulates aerobic glycolysis in prostate cancer." in: Cancer research, Vol. 73, Issue 6, pp. 1900-11, (2013) (PubMed).

Zhang, Li, Jaiswal, Bayat, Xiong, Sandoval, Charng, David, Haueter, Yamamoto, Graham, Bellen: "The C8ORF38 homologue Sicily is a cytosolic chaperone for a mitochondrial complex I subunit." in: The Journal of cell biology, Vol. 200, Issue 6, pp. 807-20, (2013) (PubMed).

Target Details for V5 Epitope Tag

Target: V5 Epitope Tag

Alternative Name: V5-Tag (V5 Epitope Tag Products)

Target Type: Tag

Background: Rabbit Anti-V5-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. Rabbit Anti-V5-tag [HRP] polyclonal antibody is suitable for detecting fusion proteins that contain V5-tags.