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His Tag antibody (HRP)

WB, ELISA Host: Mouse Monoclonal 6G2A9 HRP
Catalog No. ABIN387701
  • Target See all His Tag Antibodies
    His Tag
    Reactivity
    Please inquire
    Host
    • 92
    • 56
    • 12
    • 9
    • 8
    • 7
    • 2
    • 1
    Mouse
    Clonality
    • 105
    • 69
    • 10
    Monoclonal
    Conjugate
    • 91
    • 21
    • 14
    • 12
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This His Tag antibody is conjugated to HRP
    Application
    • 152
    • 102
    • 38
    • 27
    • 25
    • 17
    • 14
    • 14
    • 14
    • 13
    • 13
    • 7
    • 7
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Brand
    THE™
    Sequence
    HHHHHH
    Specificity
    THETM His Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
    Characteristics
    This product is suitable for detecting the expression level of His fusion proteins or His by various immunoassays.
    Purification
    Protein A affinity column
    Immunogen
    A synthetic peptide HHHHHH coupled - KLH
    Clone
    6G2A9
    Isotype
    IgG
    Top Product
    Discover our top product His Tag Primary Antibody
  • Application Notes
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.05-0.5 µg/mL
    Western blot: 0.1-1.0 µg/mL
    Other applications: user-optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    0.5 mg/mL
    Buffer
    PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing cycles.
    Storage
    4 °C/-20 °C
    Storage Comment
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Samelson, Gore, Whiting, Nygren, Purkey, Colledge, Langeberg, DellAcqua, Zweifel, Scott: "A-kinase Anchoring Protein 79/150 Recruits Protein Kinase C to Phosphorylate Roundabout Receptors." in: The Journal of biological chemistry, Vol. 290, Issue 22, pp. 14107-19, (2015) (PubMed).

    Almeida, Abreu, Matos, Fraga, Fernandes, Macedo, Gutiérrez-Gallego, Pereira, Carvalho, Macedo-Ribeiro: "SUMOylation of the brain-predominant Ataxin-3 isoform modulates its interaction with p97." in: Biochimica et biophysica acta, Vol. 1852, Issue 9, pp. 1950-9, (2015) (PubMed).

    Isik, Sliepen, van Montfort, Sanders: "Enhanced immunogenicity of HIV-1 envelope gp140 proteins fused to APRIL." in: PLoS ONE, Vol. 9, Issue 9, pp. e107683, (2014) (PubMed).

    Vire, Skarzynski, Thomas, Nelson, David, Aue, Burke, Rader, Wiestner: "Harnessing the fc? receptor for potent and selective cytotoxic therapy of chronic lymphocytic leukemia." in: Cancer research, Vol. 74, Issue 24, pp. 7510-20, (2014) (PubMed).

    Melnikow, Xu, Liu, Bell, Ghedin, Unnasch, Lustigman: "A potential role for the interaction of Wolbachia surface proteins with the Brugia malayi glycolytic enzymes and cytoskeleton in maintenance of endosymbiosis." in: PLoS neglected tropical diseases, Vol. 7, Issue 4, pp. e2151, (2013) (PubMed).

    Jarmander, Gustavsson, Do, Samuelson, Larsson: "A dual tag system for facilitated detection of surface expressed proteins in Escherichia coli." in: Microbial cell factories, Vol. 11, pp. 118, (2012) (PubMed).

  • Target
    His Tag
    Abstract
    His Tag Products
    Target Type
    Tag
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