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LGR5 antibody (AA 250-550)

LGR5 Reactivity: Human, Mouse WB, FACS, IHC, IF, IP Host: Mouse Monoclonal 2A2 unconjugated
Catalog No. ABIN2724692
  • Target See all LGR5 Antibodies
    LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))
    Binding Specificity
    • 16
    • 15
    • 11
    • 7
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 250-550
    Reactivity
    • 103
    • 56
    • 49
    • 1
    • 1
    Human, Mouse
    Host
    • 92
    • 12
    Mouse
    Clonality
    • 82
    • 22
    Monoclonal
    Conjugate
    • 48
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This LGR5 antibody is un-conjugated
    Application
    • 77
    • 38
    • 22
    • 20
    • 16
    • 16
    • 14
    • 13
    • 9
    • 7
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
    Characteristics
    Homo sapiens leucine-rich repeat containing G protein-coupled receptor 5 (LGR5)
    Purification
    Purified from mouse ascites fluids by affinity chromatography
    Immunogen
    Human recombinant protein fragment corresponding to amino acids 250-550 of human LGR5 (NP_003658) produced in HEK293T Cell.
    Clone
    2A2
    Isotype
    IgG1
    Top Product
    Discover our top product LGR5 Primary Antibody
  • Application Notes
    WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100, IP 2 μg/500 μL
    Comment

    The concentration of the product may vary between diferrent lots.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5-1.0 mg/mL
    Buffer
    PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
  • Patel, Sharon, Baranwal, Boothello, Desai, Patel: "Heparan sulfate hexasaccharide selectively inhibits cancer stem cells self-renewal by activating p38 MAP kinase." in: Oncotarget, Vol. 7, Issue 51, pp. 84608-84622, (2018) (PubMed).

    Yang, Bavi, Wang, Roehrl: "Immuno-proteomic discovery of tumor tissue autoantigens identifies olfactomedin 4, CD11b, and integrin alpha-2 as markers of colorectal cancer with liver metastases." in: Journal of proteomics, Vol. 168, pp. 53-65, (2018) (PubMed).

    Miyake, Li, Lee, Koike, Chen, Seo, Pierro: "Liver damage, proliferation, and progenitor cell markers in experimental necrotizing enterocolitis." in: Journal of pediatric surgery, Vol. 53, Issue 5, pp. 909-913, (2018) (PubMed).

    Ma, Wang, Wang, Luo, Fan: "NANOGP8 is the key regulator of stemness, EMT, Wnt pathway, chemoresistance, and other malignant phenotypes in gastric cancer cells." in: PLoS ONE, Vol. 13, Issue 4, pp. e0192436, (2018) (PubMed).

    Liu, Zheng, Ji, Liu, Hou, Li, Li: "Resveratrol reduces senescence-associated secretory phenotype by SIRT1/NF-κB pathway in gut of the annual fish Nothobranchius guentheri." in: Fish & shellfish immunology, Vol. 80, pp. 473-479, (2018) (PubMed).

    Wang, Chen, Cao, Ma, Yin, Jia, Zang, Fan: "LGR5 Is a Gastric Cancer Stem Cell Marker Associated with Stemness and the EMT Signature Genes NANOG, NANOGP8, PRRX1, TWIST1, and BMI1." in: PLoS ONE, Vol. 11, Issue 12, pp. e0168904, (2017) (PubMed).

    Osawa, Takahashi, Nishimura, Ohta, Haraguchi, Hata, Yamamoto, Mizushima, Takemasa, Doki, Mori: "Full-length LGR5-positive cells have chemoresistant characteristics in colorectal cancer." in: British journal of cancer, Vol. 114, Issue 11, pp. 1251-60, (2016) (PubMed).

    Chen, Wei, Sun, Bhattacharya, Savage, Serda, Mackeyev, Curley, Bu, Wang, Chen, Cohen-Gould, Huang, Shen, Lipkin, Copeland, Jenkins, Shuler: "A recellularized human colon model identifies cancer driver genes." in: Nature biotechnology, Vol. 34, Issue 8, pp. 845-51, (2016) (PubMed).

    Hsu, Liu, Tseng, Yang, Yeh, You, Hung, Chen, Chen: "CBB1003, a lysine-specific demethylase 1 inhibitor, suppresses colorectal cancer cells growth through down-regulation of leucine-rich repeat-containing G-protein-coupled receptor 5 expression." in: Journal of cancer research and clinical oncology, Vol. 141, Issue 1, pp. 11-21, (2015) (PubMed).

    Shaikh, Zhou, Teo, Garg, Neogi, Figg, Yeo, Yu, Maguire, Zhao, Bennett, Azizan, Davenport, McKenzie, Brown: "LGR5 Activates Noncanonical Wnt Signaling and Inhibits Aldosterone Production in the Human Adrenal." in: The Journal of clinical endocrinology and metabolism, Vol. 100, Issue 6, pp. E836-44, (2015) (PubMed).

    Jiang, Zhou, Chen, Schiff, Lee, Kong, Embree, Zhou, Mao: "Postnatal epithelium and mesenchyme stem/progenitor cells in bioengineered amelogenesis and dentinogenesis." in: Biomaterials, Vol. 35, Issue 7, pp. 2172-80, (2014) (PubMed).

    Wang, Guo, Lui, Chu, Yoo, Chang, Yen: "Identification of a distinct small cell population from human bone marrow reveals its multipotency in vivo and in vitro." in: PLoS ONE, Vol. 9, Issue 1, pp. e85112, (2014) (PubMed).

    Hsu, Liu, Tseng, Tan, Chen, Chen: "LGR5 regulates survival through mitochondria-mediated apoptosis and by targeting the Wnt/?-catenin signaling pathway in colorectal cancer cells." in: Cellular signalling, Vol. 26, Issue 11, pp. 2333-42, (2014) (PubMed).

    Lühnsdorf, Epe, Khobta: "Excision of uracil from transcribed DNA negatively affects gene expression." in: The Journal of biological chemistry, Vol. 289, Issue 32, pp. 22008-18, (2014) (PubMed).

    Wu, Xie, Gao, Wang, Guo, Tian, Li, Fan: "Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers." in: Gene, Vol. 525, Issue 1, pp. 18-25, (2013) (PubMed).

    Pastò, Marchesi, Diamantini, Frasson, Curtarello, Lago, Pilotto, Parenti, Esposito, Agostini, Nitti, Amadori: "PKH26 staining defines distinct subsets of normal human colon epithelial cells at different maturation stages." in: PLoS ONE, Vol. 7, Issue 8, pp. e43379, (2012) (PubMed).

  • Target
    LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))
    Alternative Name
    LGR5 (LGR5 Products)
    Synonyms
    lgr5-a antibody, lgr5a antibody, FEX antibody, Gpr49 antibody, GPR49 antibody, GPR67 antibody, GRP49 antibody, HG38 antibody, leucine-rich repeat containing G protein-coupled receptor 5 L homeolog antibody, leucine rich repeat containing G protein coupled receptor 5 antibody, leucine rich repeat containing G protein-coupled receptor 5 antibody, lgr5.L antibody, Lgr5 antibody, LGR5 antibody
    Background
    LGR5 (leucine-rich repeat containing G protein-coupled receptor 5) An Orphan GPCR protein, contains large extracellular domain, Stem cell biomarker for small intestine, active in colon cancer, overexpressed in ovary and liver tumors.
    Molecular Weight
    99.8 kDa
    Gene ID
    8549
    NCBI Accession
    NM_003667
    HGNC
    8549
    Pathways
    WNT Signaling
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