VEGF antibody (AA 27-120)

Catalog No. ABIN727205

Product Details anti-VEGF Antibody

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Binding Specificity: AA 27-120

Reactivity: Human, Mouse, Rat, Pig, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Goat, Human, Mouse, Pig, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human VEGF

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-VEGF antibody (ABIN727205)

Bi, Zhang, Du, Zhang, Deng, Liu, Zhang: "Peripheral serotonin regulates postoperative intra-abdominal adhesion formation in mice." in: Scientific reports, Vol. 7, Issue 1, pp. 10001, (2019) (PubMed).

Chen, Zhao, Li, Shou, Zheng, Li, Qi, Yu: "Circulating fibrocyte mobilization in negative pressure wound therapy." in: Journal of cellular and molecular medicine, Vol. 21, Issue 8, pp. 1513-1522, (2018) (PubMed).

Varghese, Joseph, S R, B S, Sreelekha: "The inhibitory effect of anti- tumor polysaccharide from Punica granatum on metastasis." in: International journal of biological macromolecules, Vol. 103, pp. 1000-1010, (2017) (PubMed).

Yasukawa, Fujihara, Fujimori, Kawaguchi, Yamada, Nakayama, Yamamoto, Kishi, Hamada, Masutani: "Synergetic Effects of PARP Inhibitor AZD2281 and Cisplatin in Oral Squamous Cell Carcinoma in Vitro and in Vivo." in: International journal of molecular sciences, Vol. 17, Issue 3, pp. 272, (2016) (PubMed).

Wang, Hao, Shi, Pu, Wang: "Effects of minocycline on apoptosis and angiogenesis-related protein expression in a rat model of intracerebral hemorrhage." in: Neural regeneration research, Vol. 7, Issue 8, pp. 595-600, (2015) (PubMed).

Li, Dong, Wei, Wang, Zhang, Li: "Fatty acid synthase mediates the epithelial-mesenchymal transition of breast cancer cells." in: International journal of biological sciences, Vol. 10, Issue 2, pp. 171-80, (2014) (PubMed).

Zhang, Li, Li, Chang, Chen, Jiang, Zhao, Guo: "Expression and localization of the vascular endothelial growth factor and changes of microvessel density during hair follicle development of Liaoning cashmere goats." in: Genetics and molecular research : GMR, Vol. 12, Issue 4, pp. 6424-32, (2014) (PubMed).

Zeng, Li, Dong, Ma, Jiang, Jin, Tu: "Sprengerinin C exerts anti-tumorigenic effects in hepatocellular carcinoma via inhibition of proliferation and angiogenesis and induction of apoptosis." in: European journal of pharmacology, Vol. 714, Issue 1-3, pp. 261-73, (2013) (PubMed).

Ryzhov, Goldstein, Novitskiy, Blackburn, Biaggioni, Feoktistov: "Role of A2B adenosine receptors in regulation of paracrine functions of stem cell antigen 1-positive cardiac stromal cells." in: The Journal of pharmacology and experimental therapeutics, Vol. 341, Issue 3, pp. 764-74, (2012) (PubMed).

Qi, Qian, Sun, Liu, Wu, Sun: "Inhaled NO contributes to lung repair in piglets with acute respiratory distress syndrome via increasing circulating endothelial progenitor cells." in: PLoS ONE, Vol. 7, Issue 3, pp. e33859, (2012) (PubMed).

Guo, Chen, Huang, Li: "Combinatorial effects of naomai yihao capsules and vascular endothelial growth factor gene-transfected bone marrow mesenchymal stem cells on angiogenesis in cerebral ischemic tissues in rats." in: Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan, Vol. 32, Issue 1, pp. 87-92, (2012) (PubMed).

Target Details for VEGF

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Alternative Name: VEGF (VEGF Products)

Synonyms: MVCD1 antibody, VEGF antibody, VPF antibody, Vegf antibody, Vegf120 antibody, Vegf164 antibody, Vegf188 antibody, Vpf antibody, VEGF-A antibody, VEGF164 antibody, eVEGF120 antibody, eVEGF164 antibody, vegf-a antibody, vegf antibody, vascular endothelial growth factor A antibody, vascular endothelial growth factor A L homeolog antibody, vascular endothelial growth factor antibody, vascular endothelial growth factor precursor antibody, VEGFA antibody, Vegfa antibody, vegfa.L antibody, VEGF antibody, vegf antibody

Background:

Synonyms: VPF, VEGF, MVCD1, Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VEGFA

Background: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Gene ID: 7422

UniProt: P15692